Volume 14, Number 5—May 2008
Serologic Evidence for Novel Poxvirus in Endangered Red Colobus Monkeys, Western Uganda
Enzyme-linked immunosorbent assay, Western blot, and virus neutralization assays indicated that red colobus monkeys in Kibale National Park, western Uganda, had antibodies to a virus that was similar, but not identical, to known orthopoxviruses. The presence of a novel poxvirus in this endangered primate raises public health and conservation concerns.
The virus subfamily Chordopoxviridae contains emerging pathogens of considerable concern, both because of their historic impact on global human health and because of their zoonotic potential (1). Although certain poxviruses in this subfamily are among the most extensively studied viral pathogens (e.g., smallpox, vaccinia), the natural diversity of mammalian poxviruses remains poorly characterized. This fact is especially true in sub-Saharan Africa, where at least 1 zoonotic orthopoxvirus (monkeypox) has caused sporadic human outbreaks since 1970 (2), and infected rodents exported to the United States caused a highly publicized human outbreak in 2003 (3,4). In this study, we describe serologic evidence for a previously uncharacterized poxvirus in endangered red colobus monkeys (Procolobus rufomitratus tephrosceles) from Kibale National Park, western Uganda (5,6). Our results, based on postadsorption ELISA, Western blot, and virus neutralization assays, extend our understanding of the host range and diversity of poxviruses and raise public health and conservation concerns.
From June 12 to June 24, 2006, 31 red colobus (13 males, 18 females, all adult or subadult) were sampled from Kibale National Park, western Uganda (5,6). Animals were chemically immobilized in the field by intramuscular injection of tiletamine/zolezepam (4.6–9.6 mg/kg body weight) with 1.0- or 1.5-mL plastic darts with 5/8-inch needles shot from a variable-pressure compressed air rifle. After initial darting, animals were given tiletamine/zolezepam or ketamine HCl intravenously as needed. Blood samples were collected into vacutainers containing sodium-EDTA, plasma was separated in the field by centrifugation, and samples were stored in liquid nitrogen for transport to North America. Animals were placed in cloth bags to recover from anesthesia and were released near trees and vines that were easy to climb and within visual range of their social groups. All animals appeared healthy at the time of capture. Animal protocols were approved by the McGill University Animal Care Committee before data collection.
A vaccinia virus (VV) ELISA was used as an initial screening test to detect antipoxvirus antibodies. VV-reactive antibodies were detected with horseradish peroxidase (HRP)–conjugated polyclonal goat anti–rhesus macaque (RM; Macaca mulatta) immunoglobulin (Ig) G (Fc specific; Nordic Laboratories, Tilsbug, the Netherlands), which readily detected antibodies of other primate species, including red colobus monkeys and humans. By using this approach, samples from 8 (26%) of 31 red colobus plasma scored positive (>200 ELISA units [EU]), 21 (68%) of 31 scored seronegative (<100 EU), and 2 (6%) of 31 had equivocal results (100–200 EU) (Appendix Figure).
Postadsorption ELISA tests (7) were used next to determine the specificity of the response to 1 of 3 orthopoxviruses: VV, monkeypox virus (MPV), or cowpox virus (CPV). Plasma samples were tested directly by ELISA or preadsorbed (1:30) for 30 minutes at 37°C with VV, MPV, or CPV lysate normalized to contain 6 × 108 PFU/mL before addition to virus-coated ELISA plates (Figure 1). The postadsorption ELISA test allows one to differentiate among closely related orthopoxviruses. For instance, VV-specific antibodies are best depleted by VV lysate assayed on a VV-coated ELISA plate (Figure 1, panel A). However, analysis of samples from 10 red colobus with detectable anti-VV antibody responses (Appendix Figure) did not show a clear pattern following preadsorption with VV, MPV, or CPV antigens, which suggests a similar cross-reactivity to each of these 3 orthopoxviruses.
Western blot analysis was performed to characterize more fully the antipoxvirus response of the red colobus. Two micrograms of purified MPV, VV, and CPV viral proteins were separated by 4%–20% gradient sodium dodecyl sulfate–polyacrylamide gel electrophoresis, transferred to polyvinylidene difluoride membranes (Invitrogen, Carlsbad, CA, USA), and probed with plasma (1:10,000) before addition of HRP-conjugated polyclonal goat antihuman Ig G (γ-specific, Jackson Immunolabs, Inc., West Grove, PA, USA) and chemiluminescent detection (Pierce SuperSignal West Dura Substrate, Rockville, IL, USA) (Figure 2). VV-immune human, MPV-immune human, MVA (modified vaccinia Ankara)-immune RM and MPV-immune RM plasma samples were included as positive controls to identify banding patterns typically observed in orthopoxvirus-immune humans and nonhuman primates. Western blot analysis proved more sensitive than anti-VV ELISA (Appendix Figure), with plasma from 30 of 31 red colobus reacting with at least 1 protein band from MPV, VV, or CPV. However, unlike the orthopoxvirus-immune human and RM controls, samples from red colobus demonstrated fewer immunoreactive bands and different immunodominant banding patterns, suggesting infection with either a distantly related orthopoxvirus or a virus from a different genus in the Poxviridae family.
Members of the Orthopoxvirus genus elicit cross-neutralizing antibodies against other members of the same genus. To determine if the red colobus were infected with an orthopoxvirus or a more distantly related poxvirus, plaque reduction neutralization assays were performed using 100 PFU of MPV, VV, or CPV. Plasma samples from all 31 red colobus were tested, and all exhibited a neutralizing titer 50 of <20 against MPV, VV, or CPV (data not shown). These findings suggest that although the monkeys were infected with a poxvirus with serologic cross-reactivity to VV (Appendix Figure), lack of a detectable neutralizing antibody response (<20) indicates that the animals may have been infected with a poxvirus that is not a member of the Orthopoxvirus genus.
Our results provide evidence that red colobus in Kibale National Park have been exposed to a previously uncharacterized poxvirus. Kibale red colobus may have been exposed to monkeypox or to a “monkeypox-like” virus, but we could not confirm this with our current serologic tools. On the other hand, other poxviruses, such as Tanapox virus and Yaba monkey tumor virus, have been identified in Africa, and infection by 1 of these poxviruses or a related virus cannot be ruled out. Future studies will require optimizing serologic diagnostics against these divergent poxviruses (with appropriate positive and negative controls) to determine the identity of the poxvirus/poxviruses that have infected the red colobus described here. In this light, we note that tanapox, a zoonosis of suspected primate origin (8,9), derives its name from the Tana River, eastern Kenya, which supports an isolated population of red colobus closely related to those in Kibale (P. r. rufomitratus) (10,11).
A protracted outbreak of infectious disease occurred in Kibale red colobus from 1971 to 1981, where it caused a death rate up to nearly 10% in some social groups, apparently killing only adult male monkeys (12). Although neither formal clinical data nor biologic samples were collected, descriptions of lesions of affected monkeys suggested diffuse to multifocal areas of inflammation with gray mottling and epidermal crusts on the face (most commonly the lips), perineum, and inguinum, followed by alopecia and impaired locomotion. Monkeys sampled for the present study would almost certainly not yet have been born during this period, but these observations raise the possibility that outbreaks of disease at least outwardly consistent with poxvirus infection have occurred previously in Kibale red colobus.
Poxviruses are known for their potential to cross species barriers (1), and red colobus living in small, unprotected forest fragments outside of Kibale National Park interact aggressively and at high rates with local persons and their domestic animals (13). At the same time, persons in rural western Uganda already bear a high incidence of pathogens, including HIV (14), which renders a substantial proportion of the population immunocompromised and susceptible to opportunistic infections. Recent outbreaks of zoonotic poxviruses have not been documented in our study area, despite a local and regional healthcare system that would most likely have detected such events. However, the presence of a novel and potentially zoonotic poxvirus in red colobus should be viewed as a point of concern for the future of public health in this region and elsewhere.
Dr Goldberg is associate professor of epidemiology and preventive medicine in the Department of Pathobiology at the University of Illinois, Urbana-Champaign, and director of the Kibale EcoHealth Project, Uganda. His research focuses on the ecology and epidemiology of infectious diseases, with an emphasis on molecular epidemiology and zoonoses.
We gratefully acknowledge the Uganda Wildlife Authority and the Uganda National Council for Science and Technology for granting us permission to conduct this research and Makerere University Biological Field Station and the Kibale EcoHealth Project and the Kibale Fish and Monkey Project for providing logistic support. We further thank I. Messaoudi for help with sample acquisition at Oregon Health and Science University, and T. Struhsaker and D. Coleman for invaluable comments and discussion.
Oregon Health and Science University and Dr. Slifka have a financial interest in Najit Technologies, Inc., a company that may have a financial interest in the results of this research and technology. This potential conflict of interest has been reviewed and managed by Oregon Health and Science University and the Integrity Oversight Council. Support for this work was provided in part by Public Health Service grant R41 AI063675, PO1 AG023664, and Oregon National Primate Research Center grant RR000163 (to M.K.S.), Department of Defense contract W81XWH-05-1-0046 (to S.W.W.), NSERC, CFI, and CRC funding (to C.C.), and a Richard H. Tomlinson Post-Doctoral Fellowship and Wenner-Gren Foundation grant (to T.S.).
- Regnery RL. Poxviruses and the passive quest for novel hosts. Curr Top Microbiol Immunol. 2007;315:345–61.
- Di Giulio DB, Eckburg PB. Human monkeypox: an emerging zoonosis. Lancet Infect Dis. 2004;4:15–25.
- Reed KD, Melski JW, Graham MB, Regnery RL, Sotir MJ, Wegner MV, The detection of monkeypox in humans in the Western Hemisphere. N Engl J Med. 2004;350:342–50.
- Hammarlund E, Lewis MW, Carter SV, Amanna I, Hansen SG, Strelow LI, Multiple diagnostic techniques identify previously vaccinated individuals with protective immunity against monkeypox. Nat Med. 2005;11:1005–11.
- Struhsaker TT. Ecology of an African rain forest: logging in Kibale and the conflict between conservation and exploitation. Gainesville (FL): University Press of Florida; 1997.
- Chapman CA, Struhsaker TT, Lambert JE. Thirty years of research in Kibale National Park, Uganda, reveals a complex picture for conservation. Int J Primatol. 2005;26:539–55.
- Dubois MD, Slifka MK. Retrospective analysis of monkeypox infection. Emerg Infect Dis. 2008 Apr; [Epub ahead of print].
- Downie AW, Taylor-Robinson CH, Caunt AE, Nelson GS, Manson-Bahr PEC, Matthews THC. Tanapox: a new disease caused by a poxvirus. BMJ. 1971;1:363–8.
- Jezek Z, Arita I, Szceniowski M, Paluku KM, Kalisa R, Nakano JH. Human tanapox in Zaire: clinical and epidemiological observations on cases confirmed by laboratory studies. Bull World Health Organ. 1985;63:1027–35.
- Struhsaker TT. Vocalizations, phylogeny and palaeogeography of red colobus monkeys (Colobus badius). Afr J Ecol. 1981;19:265–83.
- Ting N. Mitochondrial relationships and divergence dates of the African colobines: evidence of miocene origins for the living colobus monkeys. J Hum Evol. In press.
- Struhsaker TT. Variation in adult sex ratios of red colobus monkey social groups: implications for interspecific comparisons. In: Kappeler PM, editor. Primate males: causes and consequences of variation in group composition. Cambridge (UK): Cambridge University Press; 2000. p. 108–19.
- Goldberg TL, Gillespie TR, Rwego IB, Kaganzi C. Killing of a pearl-spotted owlet (Glaucidium perlatum) by male red colobus monkeys (Procolobus tephrosceles) in a forest fragment near Kibale National Park, Uganda. Am J Primatol. 2006;68:1007–11.
- Joint United Nations Programme on HIV/AIDS (UNAIDS). AIDS epidemic update. Geneva: The Programme; 2007.
Suggested citation for this article: Goldberg TL, Chapman CA, Cameron K, Saj T, Karesh WB, Wolfe N, et al. Serologic evidence for novel poxvirus in endangered red colobus monkeys, western Uganda. Emerg Infect Dis [serial on the Internet] 2008 May [date cited]. Available from: http://wwwnc.cdc.gov/eid/article/14/5/07-1686
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