Skip directly to local search Skip directly to A to Z list Skip directly to navigation Skip directly to site content Skip directly to page options
CDC Home

Volume 14, Number 8—August 2008

Research

Diverse Contexts of Zoonotic Transmission of Simian Foamy Viruses in Asia

Lisa Jones-Engel*Comments to Author , Cynthia C. May†, Gregory A. Engel*‡, Katherine A. Steinkraus†, Michael A. Schillaci, Agustin Fuentes¶, Aida Rompis#, Mukesh K. Chalise**, Nantiya Aggimarangsee††, Mohammed M. Feeroz‡‡, Richard Grant*1, Jonathan S. Allan§§, Arta Putra#, I. Nengah Wandia#, Robin Watanabe*, LaRene Kuller*, Satawat Thongsawat††, Romanee Chaiwarith††, Randall C. Kyes*, and Maxine L. Linial†
Author affiliations: *University of Washington, Seattle, Washington, USA; †Fred Hutchinson Cancer Research Center, Seattle; ‡Swedish Hospital, Seattle; §University of Toronto, Scarborough, Toronto, Ontario, Canada; ¶Notre Dame University, Notre Dame, Indiana, USA; #Universitas Udayana, Denpasar, Bali, Indonesia; **Tribhuvan University, Kathmandu, Nepal; ††Chiang Mai University, Chiang Mai, Thailand; ‡‡Jahangirnagar University, Savar, Dhaka, Bangladesh; §§Southwest Foundation for Biomedical Research, San Antonio, Texas, USA;

Main Article

Figure 1

Figure 1 - Western blot assays using human serum. Dilutions of human serum (lanes 2–11) or a foamy virus–-positive Macaca mulatta MBG8 (lane 1) were used to probe filter strips containing equal amounts of lysates from simian foamy virus–infected cells (from M. fascicularis; i lanes) or noninfected cells (u lanes). Individual strips were developed by using TMB reagent (3,3′,5,5′-tetramethylbenzidine; Promega, Madison, WI, USA). The positions of the viral proteins Gag and Bet are indicat

Figure 1. Western blot assays using human serum. Dilutions of human serum (lanes 2–11) or a foamy virus–-positive Macaca mulatta MBG8 (lane 1) were used to probe filter strips containing equal amounts of lysates from simian foamy virus–infected cells (from M. fascicularis; i lanes) or noninfected cells (u lanes). Individual strips were developed by using TMB reagent (3,3′,5,5′-tetramethylbenzidine; Promega, Madison, WI, USA). The positions of the viral proteins Gag and Bet are indicated. Lanes 10 and 11 show the range of reactivity seen with negative serum; lane 10 shows serum with nonspecific reactivity to proteins of approximately the same size as viral proteins; lane 11 shows serum negative for both lysates.

Main Article

1Current affilation: SNBL USA Ltd., Everett, Washington, USA.

Top of Page

 

Past Issues

Select a Past Issue:

Art in Science - Selections from Emerging Infectious Diseases
Now available for order



CDC 24/7 – Saving Lives, Protecting People, Saving Money. Learn More About How CDC Works For You…

USA.gov: The U.S. Government's Official Web PortalDepartment of Health and Human Services
Centers for Disease Control and Prevention   1600 Clifton Rd. Atlanta, GA 30333, USA
800-CDC-INFO (800-232-4636) TTY: (888) 232-6348 - Contact CDC–INFO