Diverse Contexts of Zoonotic Transmission of Simian Foamy Viruses in Asia
Lisa Jones-Engel* , Cynthia C. May†, Gregory A. Engel*‡, Katherine A. Steinkraus†, Michael A. Schillaci, Agustin Fuentes¶, Aida Rompis#, Mukesh K. Chalise**, Nantiya Aggimarangsee††, Mohammed M. Feeroz‡‡, Richard Grant*1, Jonathan S. Allan§§, Arta Putra#, I. Nengah Wandia#, Robin Watanabe*, LaRene Kuller*, Satawat Thongsawat††, Romanee Chaiwarith††, Randall C. Kyes*, and Maxine L. Linial†
Author affiliations: *University of Washington, Seattle, Washington, USA; †Fred Hutchinson Cancer Research Center, Seattle; ‡Swedish Hospital, Seattle; §University of Toronto, Scarborough, Toronto, Ontario, Canada; ¶Notre Dame University, Notre Dame, Indiana, USA; #Universitas Udayana, Denpasar, Bali, Indonesia; **Tribhuvan University, Kathmandu, Nepal; ††Chiang Mai University, Chiang Mai, Thailand; ‡‡Jahangirnagar University, Savar, Dhaka, Bangladesh; §§Southwest Foundation for Biomedical Research, San Antonio, Texas, USA;
Figure 1. Western blot assays using human serum. Dilutions of human serum (lanes 2–11) or a foamy virus–-positive Macaca mulatta MBG8 (lane 1) were used to probe filter strips containing equal amounts of lysates from simian foamy virus–infected cells (from M. fascicularis; i lanes) or noninfected cells (u lanes). Individual strips were developed by using TMB reagent (3,3′,5,5′-tetramethylbenzidine; Promega, Madison, WI, USA). The positions of the viral proteins Gag and Bet are indicated. Lanes 10 and 11 show the range of reactivity seen with negative serum; lane 10 shows serum with nonspecific reactivity to proteins of approximately the same size as viral proteins; lane 11 shows serum negative for both lysates.
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