Alison J. Rodger*† , Graham S. Cooke‡§, Rosalynn Ord¶, Colin J. Sutherland¶, and Geoffrey Pasvol*§
Author affiliations: *Northwick Park Hospital, Harrow, UK; †University College Long, London, UK; ‡Africa Centre for Health and Population Studies, Somkhele, South Africa; §Imperial College London, London, UK; ¶Hospital for Tropical Diseases, London, UK
Figure. Molecular typing of malarial parasites from 6 US travelers with falciparum malaria returning from East Africa in 2005. Plasmodium falciparum merozoite surface protein 1 (Pfmsp1) (upper gel) and Pfmsp2 (lower gel) allelic variation among isolates was determined by nested PCR and agarose gel electrophoresis. DNA markers (MW) are in lanes 1 and 13 in each gel. Two family-specific primer sets were used for each of the 2 genes. No parasites of the Ro33 allelic family of msp1 were found (data not shown). Pretreatment (day 0) isolates are shown for all patients. Posttreatment (day 1) parasite isolates are also shown for patients 2–6.
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