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Volume 14, Number 8—August 2008

Dispatch

Isolation and Molecular Characterization of Banna Virus from Mosquitoes, Vietnam

Takeshi Nabeshima*, Phan Thi Nga†, Posadas Guillermo*, Maria del Carmen Parquet*, Fuxun Yu*, Nguyen Thanh Thuy†, Bui Minh Trang†, Nguyen Tran Hien†, Vu Sinh Nam†, Shingo Inoue*, Futoshi Hasebe*, and Kouichi Morita*Comments to Author 
Author affiliations: *Nagasaki University, Nagasaki City, Japan; †National Institute of Hygiene and Epidemiology, Hanoi, Vietnam;

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Table 2

Phylogenetic clustering of Banna virus genomic RNA segments*

Strain Isolation
Segments
Location Year 1 2 3 4 5 6 7 8 9 10 11 12
Length (bp) 3747 3048 2400 2038 1716 1671 1136 1119 1101 977 867 862
Aligned area 990–3227 211–2541 108–2183 570–1748 110–760 106–1386 145–981 129–913 597–872 86–751 75–609 44–664
JKT-6423 Java, Indonesia 1980 I I I I I I I I I I I I
JKT-6969 Java, Indonesia 1981 I I NA NA NA I II I II I II I
JKT-7043 Java, Indonesia 1981 I I NA NA NA I II I II I II I
Chinese Yunnan, China 1987 III III III III III III III III III III III III
YN-6 Yunnan, China 2000 NA NA NA NA NA NA NA NA III NA NA III
BJ95-75 Beijing, China 1995 NA NA NA NA NA NA NA NA III NA NA III
02VN018b Quang Binh, Vietnam 2002 IV IV IV IV IV IV IV IV IV IV III III
02VN178b Quang Binh, Vietnam 2002 IV IV IV IV IV/V V IV/V IV V IV III III
02VN009b Ha Tay, Vietnam 2002 V V V V V V V IV/V V V V/ III V
02VN078b Ha Tay, Vietnam 2002 V V V V V V V V V V V V
02VN180b Quang Binh, Vietnam 2002 V V V V V V V V V V V V

*Length, length of each segment in the reference sequence of Banna virus (BAV) from RefSeq (NC_004198, NC_004200–NC_004204, NC_004211, NC_004217–NC_004221); aligned area, the fragment used to make the alignment and phylogenetic tree. The numbers are set following the sequences of strain JHT-6423, which is the reference strain in National Center for Biotechnology Information Reference Sequences. I, the cluster including the Javanese JKT-6423 strain; II, the cluster specific to the JKT-6969 and JKT-7043 strains; III, the cluster including Chinese BAVs; IV, the cluster including the Vietnamese 02VN018b strain; V, the cluster including the Vietnamese 02VN180b strain. NA, not available.

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