Volume 14, Number 8—August 2008
Dispatch
Identification of Residual Blood Proteins in Ticks by Mass Spectrometry Proteomics
Table. Vertebrate proteins detected by mass spectrometry in extracts of Amyblomma americanum or Ixodes scapularis flat ticks
| Protein in tryptic digest | No. ticks in which protein was detected/no. examined | ||||||
|---|---|---|---|---|---|---|---|
| A. americanum nymphs | I. scapularis nymphs | I. scapularis adults | |||||
| Fed on rabbit | Fed on sheep | Fed on rabbit | Fed on mouse | Fed on mouse | |||
| Immunoglobulin | 3/4 | 3/4 | 3/5 | 4/10 | 0/3 | ||
| Globin (α or β) | 1/4 | 3/4 | 0/5 | 0/10 | 3/3 | ||
| Histone H3 | 1/4 | 3/4 | 3/5 | 5/10 | 3/3 | ||
| Histone H2 | 3/4 | 3/4 | 4/5 | 6/10 | 3/3 | ||
| Tubulin | 1/4 | 2/4 | 5/5 | 9/10 | 3/3 | ||
| Keratin | 0/4 | 3/4 | 5/5 | 7/10 | 1/3 | ||
| Actin | 1/4 | 3/4 | 5/5 | 10/10 | 0/3 | ||
| Cytochrome c-type heme lyase | 0/4 | 0/4 | 5/5 | 10/10 | 3/3 | ||
| >1 of above | 4/4 | 4/4 | 5/5 | 10/10 | 3/3 | ||
