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Volume 14, Number 8—August 2008

Dispatch

Identification of Residual Blood Proteins in Ticks by Mass Spectrometry Proteomics

Samanthi Wickramasekara*1, Jonas Bunikis†1, Vicki Wysocki*, and Alan G. Barbour†Comments to Author 
Author affiliations: *University of Arizona, Tucson, Arizona, USA; †University of California Irvine, Irvine, California, USA;

Main Article

Table. Vertebrate proteins detected by mass spectrometry in extracts of Amyblomma americanum or Ixodes scapularis flat ticks

Protein in tryptic digestNo. ticks in which protein was detected/no. examined
A. americanum nymphs

I. scapularis nymphs
I. scapularis adults
Fed on rabbitFed on sheepFed on rabbitFed on mouseFed on mouse
Immunoglobulin3/43/43/54/100/3
Globin (α or β)1/43/40/50/103/3
Histone H31/43/43/55/103/3
Histone H23/43/44/56/103/3
Tubulin1/42/45/59/103/3
Keratin0/43/45/57/101/3
Actin1/43/45/510/100/3
Cytochrome c-type heme lyase
0/4
0/4

5/5
10/10

3/3
>1 of above4/44/45/510/103/3

Main Article

1These authors contributed equally to this article.

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