Volume 15, Number 1—January 2009
Dispatch
Rickettsia helvetica in Dermacentor reticulatus Ticks
Marinko Dobec
, Dragutin Golubic, Volga Punda-Polic, Franz Kaeppeli , and Martin Sievers
, Dragutin Golubic, Volga Punda-Polic, Franz Kaeppeli , and Martin SieversTable 1
Primers and probes designed for real-time PCR*
| Species | Primers and probe | Sequence (5′ → 3′) |
|---|---|---|
| Rickettsia helvetica | ompB_forward | GATTTCGACGGTAAAATTACC |
| ompB_reverse | GCTACCGATATTACCTACAG | |
| ompB_probe† |
ACTCTACTGCTACAAGTATGGTTGCTACAG |
|
| R. slovaca | ompA_forward | GTGATAATGTTGGCAATAATAATTGG |
| ompA_reverse | CTCTCGTTATAATCGAACCAAC | |
| ompA_probe† |
CAGCAGGAGTACCATTAGCTACCCCTCC |
|
| Dermacentor spp. | ITS_forward | GTGCGTCCGTCGACTCGTTTTGA |
| ITS_reverse | ACGGCGGACTACGACGGAATGC |
*Amplified products: 162 bp (R. helvetica); 228 bp (R. slovaca); 646 bp (D. reticulatus); standard curve: slope; Y-intercept and correlation coefficient:
–3.634, 40.129, 0.9937 (R. helvetica); –0.23, 42.82, 0.9942 (R. slovaca); ITS, internal transcribed spacer.
†The TaqMan probes were labeled with the fluorescent dyes FAM at the 5' end and TAMRA as quencher at the 3' end.
