Volume 15, Number 1—January 2009
Dispatch
Isolation of Candidatus Bartonella melophagi from Human Blood1
Table
Sequence similarities for 16S–23S ITS and 3 genes from 2 patient isolates and available GenBank sequences*
| Sequence or gene | Basepair homology (%) | Basepair homology (%) |
|---|---|---|
| ITS | Bartonella sp. tick† | Bartonella melophagi‡ |
| Patient 1 | 405/408 (99.3) | 385/388 (99.2) |
| Patient 2 |
405/408 (99.3) |
385/388 (99.2) |
| gltA | Bartonella sp. sheep blood§ | B. melophagi¶ |
| Patient 1 |
131/134 (97.8) |
183/187 (97.9) |
| rpoB | B. melophagi# | |
| Patient 1 |
NA |
651/656 (99.2) |
| 16S rRNA | Wolbachia melophagi** | B. melophagi†† |
| Patient 1 | 670/671 (99.8) | 631/633 (99.7) |
*ITS, internal transcribed spacer; gltA, citrate synthase; rpoB, RNA polymerase B; NA, not available.
†Uncultured Bartonella sp. clone BT7498 sequenced from a tick from Peru (GenBank accession no. AF415209).
‡Candidatus B. melophagi strain K-2C isolated from a sheep ked (M. Kosoy, unpub. data).
§Bartonella sp. isolated from commercial sheep blood agar plates (GenBank accession no. EU020109).
¶Candidatus B. melophagi strains K-9B and K-2C isolated from sheep ked (GenBank accession nos. AY724769 and AY724769).
#Candidatus B. melophagi strain K-2C isolated from sheep ked (GenBank accession no. EF605288).
**W. melophagi sequenced from Melophagus ovinus sheep keds (GenBank accession no. X89110).
††Candidatus B. melophagi strain K-2C isolated from a sheep ked (GenBank accession no. AY724770).
