Volume 15, Number 10—October 2009
Dispatch
Escherichia coli as Reservoir for Macrolide Resistance Genes
Minh Chau Phuc Nguyen, Paul-Louis Woerther, Mathilde Bouvet, Antoine Andremont, Roland Leclercq
, and Annie Canu
, and Annie CanuTable 2
Oligonucleotide primers used for detection of Escherichia coli macrolide resistance genes
| Target gene | Primer | Sequence, 5′ → 3′ | Product size, bp | Annealing temperature, oC |
|---|---|---|---|---|
| mph(A) | mphAF | GTGAGGAGGAGCTTCGCGAG | 403 | 60 |
| mphAR |
TGCCGCAGGACTCGGAGGTC |
|||
| mph(B) | mphBF | GATATTAAACAAGTAATCAGAATAG | 494 | 58 |
| mphBR |
GCTCTTACTGCATCCATACG |
|||
| erm(A) | ermAF | TCTAAAAAGCATGTAAAAGAAA | 533 | 52 |
| ermAR |
CGATACTTTTTGTAGTCCTTC |
|||
| erm(B) | ermBF | GAAAAAGTACTCAACCAAATA | 639 | 45 |
| ermBR |
AATTTAAGTACCGTTACT |
|||
| erm(C) | ermCF | TCAAAACATAATATAGATAAA | 642 | 45 |
| ermCR |
GCTAATATTGTTTAAATCGTCAAT |
|||
| ere(A) | ereAF | GCCGGTGCTCATGAACTTGAG | 420 | 60 |
| ereAR |
CGACTCTATTCGATCAGAGGC |
|||
| ere(B) | ereBF | TTGGAGATACCCAGATTGTAG | 537 | 55 |
| ereBR |
GAGCCATAGCTTCAACGC |
|||
| mef(A) | mefAF | AGTATCATTAATCACTAGTGC | 345 | 54 |
| mefAR |
TTCTTCTGGTACTAAAAGTGG |
|||
| msr(A) | msrAF | GCACTTATTGGGGGTAATGG | 384 | 58 |
| msrAR | GTCTATAAGTGCTCTATCGTG |
