Figure 1. A) Electron micrograph of adenovirus particles isolated from Pipistrellus pipistrellus bat 199/07, Germany. Negatively stained with 1% uranyl acetate. Virus particles were 70–90 nm in diameter with an icosahedral shape. Scale bar = 100 nm. B) Schematic representation of the genomic fragments obtained from bat adenovirus 2 (GenBank accession no. FJ983127) in correspondence to canine adenovirus 2 strain Toronto A26/61 (GenBank accession no. U77082). Genomic fragments were generated by generic adenovirus-specific PCR (10) and a virus discovery based on cDNA–amplified fragment length polymorphism PCR method (11). Partial sequence of the DNA polymerase gene was generated from LongRange PCR product. Purified PCR products were directly sequenced by using the BigDye Terminator Cycle Sequencing ready Reaction kit (Applied Biosystems, Foster City, CA, USA) and analyzed on an ABI 3770 automatic sequencer (Applied Biosytems). C, clone; P, ≈550-bp nested PCR product.
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