Figure 1. Unrooted neighbor-joining tree based on 23 variable number tandem repeat loci demonstrating that the Punjab isolates (black text and PRL-20) are genetically distinct from and less diverse than available whole genome sequences (WGS, red text) (8). Statistical supports for branches based on 1,000 bootstrap iterations are shown. Sample PRL-20 is shown in red text because it is also available as a whole genome sequence; therefore, it was used in all 3 situations where an average pairwise distance (APD) was calculated. Among 10 WGS, the average pairwise distance was 0.607; between 10 WGS and Punjab isolates, average pairwise distance was 0.627; and among 15 Punjab isolates, average pairwise distance was 0.312. These results indicate that the Punjab isolates are more closely related to each other than to the sequenced strains because the APD among Punjab isolates is 2× lower than the APD calculated in the other 2 situations.