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Volume 15, Number 12—December 2009

Research

Novel Calicivirus Identified in Rabbits, Michigan, USA

Ingrid L. BerginComments to Author , Annabel G. Wise, Steven R. Bolin, Thomas P. Mullaney, Matti Kiupel, and Roger K. Maes
Author affiliations: University of Michigan, Ann Arbor, Michigan, USA (I.L. Bergin); Michigan State University, Lansing, Michigan, USA (A.G. Wise, S.R. Bolin, T.P. Mullaney, M. Kiupel, R.K. Maes)

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Table

Primers used in genomic sequencing of Michigan rabbit calicivirus*

Genomic region(s) spanned Forward and reverse primer pairs† Nucleotide positions in RHDV‡
Polymerase-capsid (VP60) 5′-ATGCCATGACTCCGATGATGGT-3′ (+) R 4835–4856

5′-CTTGTTGGTCCACCTGTTG-3′ (−) M
5482–5500
Polymerase 5′- GCGACTTCTTGTGCTTGGACTAC-3′ (+) R 4493–4515

5′- GCACCACTCCAACTGTCTGAGAA-3′ (−) M
5027–5049
Polymerase 5′-GTGACCCAGACAGTGACAAGT-3′ (+) R 3929–3949

5′-GGCCTATTTCTGCACATGCTT-3′ (−) M
4754–4774
Protease-polymerase 5′-GCGGTGACCARGGTGTTGATG-3′ (+) R 2819–2839

5′-GCCGCAGCACGCTCTATGAAT-3′ (−) M
4020–4040
VPg-protease 5′-AACAAAGCCGTTGAAAGTTGG-3′ (+) R 1927–1947

5′-TGGCAGCTCTGTTCTTCATTT-3′ (−) M
3119–3139
Helicase 5′-GAGGTTGTTTGACACGTTTGA-3′ (+) R 1089–1109

5′-TGTCATATTCACACAGCCCAG -3′ (−) M
2398–2418
Capsid (VP60)–ORF 2 5′-GTTACGACAGTGCAGGCCTATG-3′ (+) M 6412–6433
5′-CTCGCCAGTGGTGTTATAAATC -3′ (−) R 7345–7366

*RHDV, rabbit hemorrhagic disease virus; MRCV, Michigan rabbit calicivirus; VP, viral protein; ORF, open reading frame.
†RHDV-specific primer denoted by R; MRCV-specific primer denoted by M.
‡Positions spanned by primer set in RHDV genome (Italy BS89 strain, GenBank accession no. X87607).

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