Figure. Microscopic appearance of Babesia sp. EU1 sporozoites isolated from tick salivary glands and of subsequent asexual development in erythrocytes. Sporozoites were stained with Giemsa and observed in the suspension of crushed salivary glands (A, B) and from salivary glands directly crushed between slides (C, D, E). Arrows indicate sporozoite dividing forms. A composite panel of asexual stages cultivated in sheep erythrocytes from these sporozoites is presented (F); developmental stages are indicated by letters (D, dividing stages; M, free merozoites; S, schizont-like form; T, trophozoite). Scale bars = 5 μm.