Volume 15, Number 3—March 2009
Dispatch
Rickettsia spp. in Ticks, Poland
Tomasz Chmielewski
, Edyta Podsiadly, Grzegorz Karbowiak, and Stanislawa Tylewska-Wierzbanowska
, Edyta Podsiadly, Grzegorz Karbowiak, and Stanislawa Tylewska-WierzbanowskaTable 1
Oligonucleotide primers used for PCR amplification and sequencing of rickettsial species in ticks, Poland, 2005–2007
| No. | Primers | Fragment gene (size, bp) | Nucleotide sequences (5′ → 3′) | Reference |
|---|---|---|---|---|
| 1 | RpCS.409d | Citrate synthase
(850) |
CCTATGGCTATTATGCTTGC | (5) |
| RpCS.1258n |
ATTGCAAAAAGTACAGTGAACA |
|||
| 2 | Rr190-70 | Outer membrane protein A
(632) |
ATGGCGAATATTTCTCCAAAA | (3) |
| Rr190-701 |
GTTCCGTTAATGGCAGCATCT |
|||
| 3 | Rr17.61p | 17-kDa genus-common antigen (434) | GCTCTTGCA ACT TCT ATG TT | (7) |
| Rr17.492n | CATTGTTCGTCAGGTTGGCG |
