Emerging Infectious Disease ISSN: 1080-6059

Spotted Fever Group Rickettsia sp. Closely Related to R. japonica, Thailand

Nobuhiro Takada

, Hiromi Fujita, Hiroki Kawabata, Shuji Ando, Akiko Sakata, Ai Takano, and Udom Chaithong

Author affiliations: University of Fukui, Fukui, Japan (N. Takada); Ohara General Hospital, Fukushima, Japan (H. Fujita); National Institute of Infectious Diseases, Tokyo, Japan (H. Kawabata, S. Ando, A. Sakata, A. Takano); Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand (U. Chaithong)

Main Article

Figure

Phylogenetic analysis based on ompA gene (A) and rickettsial genus–specific 17-kDa antigen gene (B). Sequences were aligned by using the ClustalW software package (http://clustalw.ddbj.nig.ac.jp/top-j.html), and neighbor-joining phylogenetic tree construction and bootstrap analysis were conducted according to the Kimura 2-parameter method (www.ddbj.nig.ac.jp). Pairwise alignments were performed with an open-gap penalty of 10, a gap extension penalty of 0.5, and a gap distance of 8. Multiple alig

Figure. Phylogenetic analysis based on ompA gene (A) and rickettsial genus–specific 17-kDa antigen gene (B). Sequences were aligned by using the ClustalW software package (http://clustalw.ddbj.nig.ac.jp/top-j.html), and neighbor-joining phylogenetic tree construction and bootstrap analysis were conducted according to the Kimura 2-parameter method (www.ddbj.nig.ac.jp). Pairwise alignments were performed with an open-gap penalty of 10, a gap extension penalty of 0.5, and a gap distance of 8. Multiple alignments were also performed with the same values, and the phylogenetic branches were supported by bootstrap analysis with 1,000 replications (>800 were indicated). Rickettsia felis (CP000053) and R. canadensis (CP000409) were used as outgroups for ompA and 17-kDa antigen gene, respectively. The phylogenetic tree was constructed by using TreeView software version 1.5 (http://taxonomy.zoology.gla.ac.uk/rod/treeview.html). Scale bars indicate nucleotide substitutions (%) per site.

Main Article

Top of Page

The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.