Emerging Infectious Disease ISSN: 1080-6059

Concurrent Chikungunya and Dengue Virus Infections during Simultaneous Outbreaks, Gabon, 2007

Eric M. Leroy

, Dieudoné Nkoghe, Benjamin Ollomo, Chimène Nze-Nkogue, Pierre Becquart, Gilda Grard, Xavier Pourrut, Rémi N. Charrel, Grégory Moureau, Angélique Ndjoyi-Mbiguino, and Xavier de Lamballerie

Author affiliations: Centre International de Recherches Médicales de Franceville, Franceville, Gabon (E.M. Leroy, D. Nkoghe, B. Ollomo, C. Nze-Nkogue, P. Becquart, G. Grard, X. Pourrut); Université de la Méditerranée, Marseille, France (R. Charrel, G. Moureau, X. De Lamballerie); Université des Sciences de la Santé, Libreville, Gabon (A. Ndjoyi-Mbiguino)

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Figure 2

Phylogenetic relationships among dengue-2 virus (DENV-2) isolates based on full-length sequences (10,695 nt). A total of 85 DENV-2 genomes were compared with the human isolate obtained during the Gabon outbreak. Phylogeny was inferred by using neighbor-joining analysis. A neighbor-joining tree was constructed by using MEGA version 3.2 (www.megasoftware.net) with the Kimura 2-parameter corrections of multiple substitutions. Reliability of nodes was assessed by bootstrap resampling with 1,000 repl

Figure 2. Phylogenetic relationships among dengue-2 virus (DENV-2) isolates based on full-length sequences (10,695 nt). A total of 85 DENV-2 genomes were compared with the human isolate obtained during the Gabon outbreak. Phylogeny was inferred by using neighbor-joining analysis. A neighbor-joining tree was constructed by using MEGA version 3.2 (www.megasoftware.net) with the Kimura 2-parameter corrections of multiple substitutions. Reliability of nodes was assessed by bootstrap resampling with 1,000 replicates. Branches are scaled according the number of substitutions per site, and the branch leading to the Thailand 94 strain was shortened for convenience. Bootstrap values are shown for major key nodes.

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