Volume 15, Number 5—May 2009
Research
CTX-M β-Lactamases in Escherichia coli from Community-acquired Urinary Tract Infections, Cambodia
Etienne Ruppé
, Sopheak Hem, Sovannarith Lath, Valérie Gautier, Frédéric Ariey, Jean-Louis Sarthou, Didier Monchy, and Guillaume Arlet
, Sopheak Hem, Sovannarith Lath, Valérie Gautier, Frédéric Ariey, Jean-Louis Sarthou, Didier Monchy, and Guillaume ArletTable 1
Primers used to study CTX-M β-lactamases in Escherichia coli, Cambodia, 2004–2005
| Gene detected | Primer name | Primer sequence (5′ → 3′) | Reference |
|---|---|---|---|
| blaTEM | C | TCG GGG AAA TGT GCG CG | (23) |
| D |
TGC TTA ATC AGT GAG GCA CC |
||
| blaSHV | OS-5 | TTA TCT CCC TGT TAG CCA CC | (24) |
| OS-6 |
GAT TTG CTG ATT TCG CTC GG |
||
| blaCTX-M | MA-1 | SCS ATG TGC AGY ACC AGT AA | (23) |
| MA-2 |
CCG CRA TAT GRT TGG TGG TG |
||
| blaCTX-M group 9 | M9U | ATG GTG ACA AAG AGA GTG CA | (23) |
| M9L |
CCC TTC GGC GAT GAT TCT C |
||
| blaCTX-M group 1 | M13U | GGT TAA AAA ATC ACT GCG TC | (23) |
| M13L |
TTG GTG ACG ATT TTA GCC GC |
||
| blaampC | AmpC1 | AAT GGG TTT TCT ACG GTC TG | (25) |
| AmpC2 |
GGG CAG CAA ATG TGG AGC AA |
||
| blaVEB | casF | CGA CTT CCA TTT CCC GAT GC | (26) |
| casB |
GGA CTC TGC AAC AAA TAC GC |
||
| blaOXA-1 | OXA-1up | TAT CAA CTT CGC TAT TTT TTT A | (27) |
| OXA-1low |
TTT AGT GTG TTT AGA ATG GTG A |
||
| blaCMY | CF1 | ATGATGAAAAAATCGTTATGC | (28) |
| CF2 |
TTGTAGCTTTTCAAGAATGCGC |
||
| chuA | ChuA.1 | GAC GAA CCA ACG GTC AGG AT | (29) |
| ChuA.2 |
TGC CGC CAG TAC CAA AGA CA |
||
| ygaA | YgaA.1 | TGA AGT GTC AGG AGA CGC TG | (29) |
| YgaA.2 |
ATG GAG AAT GGG TTC CTC AAC |
||
| TspE4C2 | TspE4C2.1 | GAG TAA TGT CGG GGC ATT CA | (29) |
| TspE4C2.2 | CGC GCC AAC AAA GTA TTA GC |
