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Volume 15, Number 8—August 2009


Legionella pneumophila in Rainwater on Roads

Ryota Sakamoto, Akira OhnoComments to Author , Toshitaka Nakahara, Kazunari Satomura, Suketaka Iwanaga, Yuuichiro Kouyama, Fumiaki Kura, Naoyuki Kato, Kozo Matsubayashi, Kiyohito Okumiya, and Keizo Yamaguchi
Author affiliations: Kyoto University, Kyoto, Japan (R. Sakamoto, T. Nakahara, K. Satomura, S. Iwanaga, K. Matsubayashi); Toho University School of Medicine, Tokyo, Japan (A. Ohno, Y. Kouyama, N. Kato, K. Yamaguchi); National Institute of Infectious Diseases, Tokyo (F. Kura); Research Institute for Humanity and Nature, Kyoto (K. Okumiya)

Main Article


Legionella spp. and Acanthamoeba spp. isolated from asphalt road sources, Tokyo, Japan, July–October 2007

SourcePositive samples, no. (%)
Primary survey
Puddles on roads (n = 18)
L. pneumophila*7 (38.9)
Acanthamoeba spp.†4 (22.2)
Rainwater (n = 10)
L. pneumophila*0
Legionella spp.‡1 (10.0)
Acanthamoeba spp.†3 (30.0)
Swabs from roads on sunny days (n = 12)
L. pneumophila*0
Legionella spp.‡2 (16.7)
Acanthamoeba spp.†
2 (16.7)
Fixed-point observation of puddles on roads
L. pneumophila concentration (n = 45)
<20 CFU/100 mL29 (64.4)
20–99 CFU/100 mL7 (15.6)
100–999 CFU/100 mL7 (15.6)
>1,000 CFU/100 mL2 ( 4.4)
L. pneumophila serogroups (n = 150 strains isolated)
156 (37.3)
350 (33.3)
215 (10.0)
612 ( 8.0)
511 ( 7.3)
Others6 ( 4.0)

*Tested by using culture method.
†Tested by using PCR method.
‡Tested by using loop-mediated isothermal amplification method.

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