Volume 15, Number 9—September 2009
Dispatch
Monitoring of Putative Vectors of Bluetongue Virus Serotype 8, Germany
Bernd Hoffmann, Burkhard Bauer, Christian Bauer, Hans-Joachim Bätza, Martin Beer, Peter-Henning Clausen, Martin Geier, Jörn M. Gethmann, Ellen Kiel, Gabriele Liebisch, Arndt Liebisch, Heinz Mehlhorn, Günter A. Schaub, Doreen Werner, and Franz J. Conraths
Table 1
Genetic characterization of batches of midges of the Culicoides obsoletus group, Germany, April 2007–May 2008*
| Batch no. | rRT-PCR, Ct | C. obsoletus sensu stricto | C. scoticus | C. chiopterus | C. dewulfi |
|---|---|---|---|---|---|
| 270/38F | 20.10 | +++ | – | +++ | +++ |
| 276/2T | 20.74 | +++ | – | +++ | +++ |
| 306/12FN | 21.33 | +++ | – | +++ | +++ |
| 304/34B | 21.60 | +++ | – | +++ | – |
| 276/2O | 23.40 | +++ | – | +++ | +++ |
| 296/52Q | 23.87 | +++ | – | +++ | +++ |
| 296/44G | 24.00 | +++ | – | +++ | +++ |
| 296/22R | 25.90 | +++ | – | +++ | +++ |
| 296/52AL | 27.26 | +++ | – | +++ | +++ |
| 306/1X | 27.30 | +++ | – | – | – |
| 306/1A | 27.60 | +++ | – | – | – |
| 306/1E | 27.70 | +++ | – | – | – |
| 306/1Z | 27.70 | +++ | – | – | – |
| 270/45B | 27.76 | +++ | – | – | +++ |
| 306/1L | 27.90 | +++ | – | – | – |
| 306/1T | 27.90 | +++ | – | – | – |
| 263/46B | 28.00 | +++ | – | ++ | +++ |
| 306/1H | 28.00 | +++ | – | – | – |
| 306/1AA | 28.00 | +++ | – | – | – |
| 306/1S | 28.10 | +++ | – | – | – |
| 306/1AC | 28.10 | +++ | – | – | – |
| 18/156B | 28.18 | +++ | – | + | +++ |
| 346/29C | 28.19 | +++ | – | – | + |
| 306/1R | 28.30 | +++ | – | – | – |
| 306/1V | 28.30 | +++ | – | – | +++ |
| 306/1N | 28.40 | +++ | ++ | – | – |
| 276/10S | 28.41 | +++ | – | +++ | +++ |
| 306/1I | 28.50 | +++ | – | – | – |
| 306/1M | 28.70 | +++ | + | +++ | – |
| 263/46A | 28.80 | +++ | – | +++ | – |
| 296/51AH | 28.82 | +++ | ++ | +++ | +++ |
| 306/1D | 28.90 | +++ | – | – | – |
| 171/13E | 29.00 | +++ | – | – | +++ |
| 306/1C | 29.00 | +++ | +++ | – | – |
| 304/1H | 29.10 | +++ | +++ | +++ | |
| 306/1B | 29.20 | +++ | – | – | – |
| 306/1F | 29.20 | +++ | – | – | – |
| 306/1U | 29.20 | +++ | – | – | – |
| 336/24B | 29.21 | +++ | – | +++ | – |
| 306/12LR | 29.26 | +++ | – | +++ | +++ |
| 306/1K | 29.30 | +++ | – | – | – |
| 306/1J | 29.40 | +++ | – | – | – |
| 270/55I | 29.47 | +++ | – | +++ | +++ |
*rRT-PCR, real-time reverse transcription–PCR; Ct, cycle threshold; –, no visible band; +, faint; ++, distinct; +++, strong band after agarose gel electrophoresis of PCR products and staining with ethidium bromide.
