Healthcare-associated Viral Gastroenteritis among Children in a Large Pediatric Hospital, United Kingdom
Nigel A. Cunliffe , J. Angela Booth, Claire Elliot, Sharon J. Lowe, Will Sopwith, Nick Kitchin, Osamu Nakagomi, Toyoko Nakagomi, C. Anthony Hart1, and Martyn Regan
Author affiliations: University of Liverpool, Liverpool, UK (N.A. Cunliffe, J.A. Booth, O. Nakagomi, T. Nakagomi, C.A. Hart); Royal Liverpool Children’s National Health Service Foundation Trust, Liverpool (N.A. Cunliffe, C. Elliot, S.J. Lowe, C.A. Hart); Health Protection Agency NW, Liverpool (C. Elliot, W. Sopwith, M. Regan); Sanofi Pasteur MSD, Maidenhead, UK (N. Kitchin); Nagasaki University, Nagasaki, Japan (O. Nakagomi, T. Nakagomi)
Figure 1. Phylogenetic tree based on viral protein (VP) 7 nucleotide sequences of serotype G1 rotavirus strains from Royal Liverpool Children’s National Health Service Foundation Trust (Alder Hey Hospital), Liverpool, UK. For each strain the source (healthcare-associated [HA] or community-acquired [CA]), specimen number, month/year of detection, and name of the strain is indicated. Reference G1P strain Wa is included. Horizontal lengths are proportional to the genetic distance calculated with Kimura’s 2-parameter method. Scale bar shows genetic distance expressed as nucleotide substitutions per site. Bootstrap probabilities >80% (>800 of 1,000 pseudoreplicate trials) are indicated at each node. Hatched VP7 sequences are from strains whose electropherotypes shared an identical L1 pattern. The VP7 nucleotide sequences used in the tree have been deposited under the strain name and accession number (in parentheses) as follows; 06AH001 (FJ797814), 06AH005 (FJ797815), 06AH033 (FJ797816), 06AH037 (FJ797817), 06AH041 (FJ797818), 06AH043 (FJ797819), 06AH047 (FJ797820), 06AH077 (FJ797821), 06AH083 (FJ797822), 06AH099 (FJ797823), 06AH143 (FJ797824), 06AH161 (FJ797825), 06AH167 (FJ797826), 06AH175 (FJ797827), 06AH218 (FJ797828), 07AH441 (FJ797829), 07AH451 (797830), 07AH466 (FJ797831), 07AH467 (FJ797832), 07AH468 (FJ797833) , 07AH490 (FJ797834), 07AH491 (FJ797835), 07AH500 (FJ797836), 07AH506 (FJ797837), 07AH508 (FJ797838), 07AH517 (FJ797839).
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