Mhammed Touinssi, Nadège Brisbarre, Christophe Picard, Coralie Frassati, Bertrand Dussol, Rathviro Uch, Pierre Gallian, Jean-François Cantaloube, Philippe de Micco, and Philippe Biagini
Author affiliations: Université de la Méditerranée, Marseille, France (M. Touinssi, N. Brisbarre, R. Uch, P. Gallian, J.-F. Cantaloube, P. de Micco, P. Biagini); Service Immunogénétique-HLA, Etablissement Français du Sang Alpes-Méditerranée, Marseille (C. Picard, C. Frassati); and Centre de Néphrologie et de Transplantation Rénale, CHU, Marseille (B. Dussol)
Figure. Alignment of parvovirus 4 (PARV4) sequences showing the location of the 2 probes used in the real-time experiments. A) Partial sequences used for the design of probe PARV4-N: PARV4 prototype isolate (AY622943) and in-house PCR products characterized initially (GenBank accession nos. FJ883557–61). B) Examples of point mutations located on the PARV4-O recognition site identified in amplicons originating from samples positive with PARV4-N assay. Mismatches identified in the alignments are underlined. Nucleotides shown in lowercase letters correspond to 5′/3′ ends of the real-time primers. Mismatches identified in the alignments are underlined and in boldface.
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