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Volume 16, Number 11—November 2010

Letter

Vibrio cholerae O1 Variant with Reduced Susceptibility to Ciprofloxacin, Western Africa

Suggested citation for this article

To the Editor: Many variants of choleragenic vibrios have emerged since the beginning of the seventh pandemic, indicating continuous evolution of this pathogenic agent. Variations occur mainly in genetic determinants of virulence and antimicrobial drug susceptibility. In September–October 2009, concurrent outbreaks of acute watery diarrhea in northeastern Nigeria (4,559 cases) and northern Cameroon (696 cases) were investigated by state ministries of health. We report reduced sensitivity to ciprofloxacin in Vibrio cholerae O1 strains and the atypical cholera toxin B (ctxB) genotype of these strains.

In September–October 2009, stool specimens from patients in Nigeria were collected on filter paper, moistened with sterile physiologic saline, and sent at room temperature to the National Reference Center for Vibrios and Cholera at the Institut Pasteur (Paris, France). Ten V. cholerae O1 biotype El Tor serotype Ogawa strains were isolated and identified by using standard procedures. Concurrently in Cameroon, 9 V. cholerae O1 Ogawa strains isolated from patient stool samples by the bacteriology laboratory of the Pasteur Center (Garoua, Cameroon) were sent to the National Reference Center for Vibrios and Cholera.

All strains were tested for antimicrobial susceptibility by MIC determination to tetracycline, trimethoprim/sulfamethoxazole, sulfonamides, ampicillin, chloramphenicol, nalidixic acid, and ciprofloxacin by using Etest (AB bioMérieux, Solna, Sweden) according to Clinical and Laboratory Standards Institute procedures and interpretative standards for V. cholerae (1). PCR amplification of the genes encoding DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE) and subsequent sequencing of PCR products were performed (2).

PCR was used to test for the presence of ctxA and ctxB genes, which encode the cholera toxin (CT), and the tcpA gene, which encodes the toxin-coregulated pilus. Genotyping of ctxB was performed by sequencing PCR products.

All isolates showed susceptibility to tetracycline (MIC 1.5 mg/L), intermediate susceptibility to ampicillin (MICs 12–16 mg/L) and chloramphenicol (MICs 8–12 mg/L), and resistance to trimethoprim/sulfamethoxazole (MIC >32 mg/L), sulfonamides (MIC >1,024 mg/L), and nalidixic acid (MIC >256 mg/L). MICs of ciprofloxacin ranged from 0.25 to 0.5 mg/L.

Sequencing of gyrA, gyrB, parC, and parE genes among all strains detected 1 mutation in gyrA (substitution of serine by isoleucine at position 83) and 1 mutation in parC (substitution of serine by leucine at position 85). Both point mutations have been associated with quinolone resistance in clinical isolates of V. cholerae (2). None of the strains had any mutations in gyrB or parE.

The presence of ctxA and ctxB genes confirmed the toxigenicity of all isolates, and tcpA PCR product size and sequence identified El Tor biotype strains. The DNA sequence of ctxB was similar to that of the recently reported Orissa variant identified in India in 2007 (3). This sequence had 2 mutations resulting in histidine at position 39 and threonine at position 68 (this amino acid sequence is similar to the CT-B subunit of the reference classical strain) and a third mutation resulting in substitution of histidine by asparagine at position 20.

We report atypical El Tor strains of V. cholerae O1 and their reduced susceptibility to ciprofloxacin in Nigeria and Cameroon. Since the 1990s, atypical El Tor strains that produce classical CT have been increasingly reported from countries in Asia, where they have gradually replaced the prototype El Tor strains, but they have only been reported in 2 countries in Africa (Mozambique and Zambia) (4,5). On the basis of the CT-B subunit sequence, these variants differ from variants isolated in southern Africa and from most variants isolated in Asia by having the same modified classical CT as a strain recently isolated in Orissa in eastern India (3), which has not been reported elsewhere. These findings indicate evolution of V. cholerae O1 El Tor hybrid strains. Their presence may indicate spread of strains from eastern India to Africa (6).

The presence of CT-B variants in central or western Africa is of great concern because these strains may be more toxigenic (3). There is also concern for the strains isolated in this study because of their reduced susceptibility to ciprofloxacin. Although reduced susceptibility to fluoroquinolone is common in southern Asia (7,8), it was reported in Africa (Zimbabwe) only recently (9). Our findings, in addition to the report of Islam et al. (9), indicate that V. cholera with reduced sensitivity to a fluoroquinolone is present in southern and western Africa. These results highlight the need for continued monitoring of antimicrobial drug susceptibility and strain tracking to maintain an efficient cholera surveillance system.

Marie-Laure Quilici, Denis Massenet, Bouba Gake, Barem Bwalki, and David M. OlsonComments to Author 
Author affiliations: Institut Pasteur, Paris, France (M.-L. Quilici); Centre Pasteur Cameroun, Garoua, Cameroon (D. Massenet, B. Gake); State Epidemiologic Unit, Yola, Adamawa State, Nigeria (B. Bwalki); Doctors Without Borders/Médecins Sans Frontières, New York, New York, USA (D. M. Olson)

Acknowledgment

This study was partially supported by a grant from the French Institute for Public Health Surveillance and by the Institut Pasteur.

References

  1. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing; 18th informational supplement. CLSI document M100–S18. Wayne (PA): The Institute; 2008.
  2. Baranwal S, Dey K, Ramamurthy T, Nair GB, Kundu M. Role of active efflux in association with target gene mutations in fluoroquinolone resistance in clinical isolates of Vibrio cholerae. Antimicrob Agents Chemother. 2002;46:26768. DOIPubMed
  3. Goel AK, Jiang SC. Genetic determinants of virulence, antibiogram and altered biotype among the Vibrio cholerae O1 isolates from different cholera outbreaks in India. Infect Genet Evol. 2010;10:8159. DOIPubMed
  4. Ansaruzzaman M, Bhuiyan NA, Nair GB, Sack DA, Lucas M, Deen JL, Cholera in Mozambique, variant of Vibrio cholerae. Emerg Infect Dis. 2004;10:20579.PubMed
  5. Safa A, Sultana J, Cam PD, Mwansa JC, Kong RY. Vibrio cholerae O1 hybrid El Tor strains, Asia and Africa. Emerg Infect Dis. 2008;14:9878. DOIPubMed
  6. Safa A, Nair GB, Kong RY. Evolution of new variants of Vibrio cholerae O1. Trends Microbiol. 2010;18:4654. DOIPubMed
  7. Garg P, Chakraborty S, Basu I, Datta S, Rajendran K, Bhattacharya T, Expanding multiple antibiotic resistance among clinical strains of Vibrio cholerae isolated from 1992–7 in Calcutta, India. Epidemiol Infect. 2000;124:3939. DOIPubMed
  8. Krishna BV, Patil AB, Chandrasekhar MR. Fluoroquinolone-resistant Vibrio cholerae isolated during a cholera outbreak in India. Trans R Soc Trop Med Hyg. 2006;100:2246. DOIPubMed
  9. Islam MS, Midzi SM, Charimari L, Cravioto A, Endtz HP. Susceptibility to fluoroquinolones of Vibrio cholerae O1 isolated from diarrheal patients in Zimbabwe. JAMA. 2009;302:23212. DOIPubMed

Suggested citation for this article: Quilici M-L, Massenet D, Gake B, Bwalki B, Olson DO. Vibrio cholerae O1 variant with reduced susceptibility to ciprofloxacin, western Africa [letter]. Emerg Infect Dis [serial on the Internet]. 2010 Nov [date cited]. http://wwwnc.cdc.gov/eid/article/16/11/10-0568.htm

DOI: 10.3201/eid1611.100568

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Table of Contents – Volume 16, Number 11—November 2010

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