Volume 16, Number 11—November 2010
Letter
Yersinia pestis DNA Sequences in Late Medieval Skeletal Finds, Bavaria
Ingrid Wiechmann
, Michaela Harbeck, and Gisela Grupe
, Michaela Harbeck, and Gisela GrupeTable
Amplification results obtained from the skeletal remains of 6 persons excavated at the St. Leonhard Catholic church, Manching-Pichl, Bavaria, Germany*
| Identification no. | Sex, age at death, y/sex | Tooth sample | DNA extract† | YP12D/ YP11R‡ | YP11D/ YP10R | YP14F/ YP13R | pst-F/ pst-R | PCP-F/ PCP-R | |
|---|---|---|---|---|---|---|---|---|---|
| 17-I | ≈11/M | 1st | I | − | + | NT | NT | − | − |
| 1st | II | − | − | NT | − | NT | NT | ||
| 2nd |
I |
− |
− |
− |
− |
NT |
NT |
||
| 22 |
20–22/F |
2nd |
I |
− |
− |
− |
NT |
NT |
NT |
| 34-I |
Young adult/M |
1st |
I |
+ |
+ |
NT |
NT |
+ |
+ |
| 73-I | 20–25/M | 1st | I | (+) | (+) | NT | NT | NT | NT |
| 1st | II | − | − | − | − | NT | NT | ||
| 2nd |
I |
− |
− |
− |
− |
NT |
NT |
||
| S1-I |
≈8/M |
1st |
I |
+ |
+ |
NT |
NT |
+ |
+ |
| S4-XX | ≈15/F | 1st | I | + | + | NT | NT | + | − |
| 1st | II | + | + | + | + | + | + | ||
| 2nd | I | + | + | + | + | + | + | ||
*Extraction controls and PCR controls had no amplicons. +, amplicon; (+), weak amplicon; –, no amplicon; NT, not tested.
†I and II represent separate extracts from the same tooth.
‡Independent PCRs with the primer pair.
