Volume 16, Number 11—November 2010
Prevalence and Genetic Structures of Streptococcus pneumoniae Serotype 6D, South Korea
To determine prevalence and genetic structures of new serotype 6D strains of pneumococci, we examined isolates from diverse clinical specimens in South Korea during 1991–2008. Fourteen serotype 6D strains accounted for 10.4% of serogroup 6 pneumococci from blood, sputum, nasopharynx, and throat samples. Serotype 6D strains consisted of 3 sequence types.
Streptococcus pneumoniae is a common cause of invasive infection in infants, children, and adults. The polysaccharide capsule of S. pneumoniae is the major virulence factor that protects the organism from host phagocytosis (1). Recently, 2 new serotypes of serogroup 6 pneumococci, 6C and 6D, were genetically and biochemically characterized (2,3). Serotype 6C was identified in 2007 on the basis of its distinct binding patterns with 2 monoclonal antibodies; serotype 6C had previously been typed as 6A according to the standard quellung reaction. Serotype 6C produces glucose in the place of galactose in the 6A capsular polysaccharide and has the wciNβ gene, which is ≈200 bp shorter than the corresponding wciN gene in 6A (2,4). After the discovery and characterization of 6C through genetic and biochemical studies, a new experimental serotype, 6X1 (later named 6D), was created by mutating the critical nucleotide in the wciP gene of the 6C capsule gene locus or by inserting the wciNβ gene into the 6B capsule gene locus (3). However, this putative serotype, 6D was thought to not exist in nature until recently, when 2 studies found 6D strains in nasopharyngeal aspirates from children in Fiji during 2004–2007 (5) and in 2 nasopharyngeal aspirates from children in South Korea in 2008 (6). Although serotype 6C has only recently been described, several studies indicate that serotype 6C pneumococci have been circulating in many countries, including the United States, the Netherlands, Australia, Israel, and South Africa (7–10). However, reports of naturally occurring serotype 6D pneumococci are limited.
We investigated the prevalence of serotypes 6C and 6D in 2 collections of pneumococci isolated from clinical specimens in South Korea. We compared the genetic diversity and antimicrobial drug susceptibility patterns of the 4 serotypes, 6A, 6B, 6C, and 6D.
Of the 2 collections of pneumococcal isolates, the first consisted of 587 clinical specimens obtained from infants and children at Seoul National University Children’s Hospital, Seoul, South Korea, from May 1991 through May 2008. The second collection consisted of 225 clinical specimens obtained from adults at 2 participating hospitals in Seoul from March 2004 through August 2007. When >1 isolate was recovered from the same person, only the initial isolate was included in the study. From these 2 sample collections (n = 812), we redetermined serotypes for 134 isolates previously assigned to serogroup 6.
Serotyping was performed by using the quellung reaction with antiserum for serogroup 6, factor 6b, and factor 6c (Statens Serum Institute, Copenhagen, Denmark). To assign serotypes 6C and 6D, we screened all strains for wciNβ and wciP6B by using 2 simplex PCRs and subsequent sequencing analysis. The wciN gene was amplified with the forward primer (5106) 5′-TAC CAT GCA GGG TGG AAT GT-3′ and the reverse primer (3101) 5′-CCA TCC TTC GAG TAT TGC-3′, resulting in product sizes of 1.8 kb for serotypes 6C and 6D for the wciNβ gene (2). The wciP gene was amplified by using the forward primer 5′-AAT TTG TAT TTT ATT CAT GCC TAT ATC TGG -3′ and the reverse primer 5′-TTA GCG GAG ATA ATT TAA AAT GAT GAC TA-3′ (11). Presence of wciNβ and wciP6B was confirmed by sequencing analysis. A characteristic of 6B wciP is the presence of an A at nucleotide position 584 (according to the sequence of wciP ), which creates a codon for asparagine at residue 195 of the 6B wciP protein. Antimicrobial drug susceptibility testing, multilocus sequence typing (MLST), and eBURST analyses were performed as described (13).
Capsular swelling reactions indicated 63 serotype 6A and 61 serotype 6B strains. However, 10 strains were not distinguished by the standard method (quellung reaction) because they reacted with both factors, 6b and 6c. Sequencing analysis showed that 6 serotype 6A strains were serotype 6C according to the presence of wciNβ but the absence of wciP6B. Subsequently, 4 serotype 6B strains and 10 undistinguished strains were identified as serotype 6D on the basis of the presence of wciNβ and wciP6B.
Serotypes tested by using the molecular method were 6A (n = 53, 39.6%), 6B (n = 61, 45.5%), 6C (n = 6, 4.5%), and 6D (n = 14, 10.4%). The earliest recovery of a serotype 6D isolate was in 1996, and the earliest recovery of a serotype 6C isolate was in 1993. Two serotype 6D strains were obtained from adults, and the remaining 12 strains were obtained from infants or children. Sources of serotype 6D isolates were blood (n = 5), sputum (n = 6), nasopharynx (n = 2), and throat (n = 1) specimens (Table). All serogroup 6 isolates except a 6C strain showed multidrug resistance to at least 3 classes of antimicrobial drugs. According to MLST, 3 sequence types (STs) were found in serotype 6D pneumococci (ST189 [n = 7], ST3171 [n = 4], and ST282 [n = 3]), which fell into 2 clonal complexes according to eBURST analysis (Figure). ST189 and ST282 were closely related to clonal complex 81, which clustered with serotype 6A strains. All 4 ST3171 strains were isolated from blood. Each ST exhibited distinct antimicrobial drug susceptibility patterns and genes for macrolide resistance (Table).
We identified 14 naturally occurring serotype 6D strains among 134 serogroup 6 pneumococci collected from diverse clinical specimens in South Korea during 1991–2008. The prevalence rate of serotype 6D among serogroup 6 isolates was 10.4%, slightly higher than that of serotype 6C (4.5%). Although serotype 6D was only recently discovered, we demonstrated that serotype 6D strains have been circulating since at least 1996. Serotype 6D was identified from various clinical sources, including blood, sputum, throat swab, and nasopharynx specimens, contrasting with findings of 2 previous studies (5,6).
The genetic structures of serotype 6D pneumococci in the MLST database (www.mlst.net) were single isolates of ST4241 (Australia); ST982, ST4190, ST5085, and ST5086 (China); and 2 isolates of ST282 (South Korea). Of those, 3 strains from China (ST982, ST5085, and ST5086) were closely related to the ST3171 strain from South Korea. This cluster of serotype 6D strains was associated with serotype 6A and 6B isolates from 3 countries in Asia. A single isolate of ST4241 was related to STs associated mostly with serotype 6B, but the ST4170 strain did not seem to be linked to other STs. This study demonstrated that 7 serotype 6D strains of ST189 and 3 serotype 6D strains of ST282 were related to clonal complex 81, which had previously been associated with only serotype 6A isolated from South Korea. However, this clonal complex also included several STs associated with many other global serotypes, such as 23F, 19F, and 19A. Although the mechanism is not completely clear, available data indicate that capsular switching from serotypes 6A, 23F, 19F, or 19A to serotype 6D is possible; this switching could occur in addition to replacement of the wciNβ gene into the 6B capsule gene locus. A previous study indicated capsular switching as the possible event for formation of serotype 6C isolates (14).
In a recent study, factor 6d antiserum was validated for accurate serotyping of 6C (10) and is now commercially available, but antiserum for detection of 6D has not yet been developed. Further studies will be required to investigate the prevalence and genetic relatedness of serotype 6D pneumococci in different countries and to evaluate the effect of pneumococcal conjugate vaccine on serotype distribution.
Dr Choi is a specialist in pediatric infectious diseases and an associate professor at Seoul National University College of Medicine. Her research interests are primarily related to pediatric respiratory infections and pneumococcal diseases.
We thank Seong Yeon Lee for her excellent technical assistance.
This study was supported by the Seoul National University Hospital Research Fund (grant no. 04-2010-0970).
- Musher DM. Streptococcus pneumoniae. In: Mandell GL, Bennett JE, Dolin R, editors. Mandell, Douglas, and Bennett’s principles and practice of infectious diseases. 7th ed. Philadelphia: Churchill Livingstone Elsevier Co.; 2010. p. 2623–7.
- Park IH, Pritchard DG, Cartee R, Brandao A, Brandileone MC, Nahm MH. Discovery of a new capsular serotype (6C) within serogroup 6 of Streptococcus pneumoniae. J Clin Microbiol. 2007;45:1225–33.
- Bratcher PE, Park IH, Hollingshead SK, Nahm MH. Production of a unique pneumococcal capsule serotype belonging to serogroup 6. Microbiology. 2009;155:576–83.
- Lin J, Kaltoft MS, Brandao AP, Echaniz-Aviles G, Brandileone MC, Hollingshead SK, Validation of a multiplex pneumococcal serotyping assay with clinical samples. J Clin Microbiol. 2006;44:383–8.
- Jin P, Kong F, Xiao M, Oftadeh S, Zhou F, Liu C, First report of putative Streptococcus pneumoniae serotype 6D among nasopharyngeal isolates from Fijian children. J Infect Dis. 2009;200:1375–80.
- Bratcher PE, Kim KH, Kang JH, Hong JY, Nahm MH. Identification of natural pneumococcal isolates expressing serotype 6D by genetic, biochemical and serological characterization. Microbiology. 2010;156:555–60.
- Jacobs MR, Good CE, Bajaksouzian S, Windau AR. Emergence of Streptococcus pneumoniae serotypes 19A, 6C, and 22F and serogroup 15 in Cleveland, Ohio, in relation to introduction of the protein-conjugated pneumococcal vaccine. Clin Infect Dis. 2008;47:1388–95.
- Hermans PW, Blommaart M, Park IH, Nahm MH, Bogaert D. Low prevalence of recently discovered pneumococcal serotype 6C isolates among healthy Dutch children in the pre-vaccination era. Vaccine. 2008;26:449–50.
- du Plessis M, von Gottberg A, Madhi SA, Hattingh O, de Gouveia L, Klugman KP. Serotype 6C is associated with penicillin-susceptible meningeal infections in human immunodeficiency virus (HIV)–infected adults among invasive pneumococcal isolates previously identified as serotype 6A in South Africa. Int J Antimicrob Agents. 2008;32:S66–70.
- Jacobs MR, Dagan R, Bajaksouzian S, Windau AR, Porat N. Validation of factor 6d antiserum for serotyping Streptococcus pneumoniae serotype 6C. J Clin Microbiol. 2010;48:1456–7.
- Pai R, Gertz RE, Beall B. Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates. J Clin Microbiol. 2006;44:124–31.
- Mavroidi A, Godoy D, Aanensen DM, Robinson DA, Hollingshead SK, Spratt BG. Evolutionary genetics of the capsular locus of serogroup 6 pneumococci. J Bacteriol. 2004;186:8181–92.
- Choi EH, Kim SH, Eun BW, Kim SJ, Kim NH, Lee J, Streptococcus pneumoniae serotype 19A in children, South Korea. Emerg Infect Dis. 2008;14:275–81.
- Jacobs MR, Bajaksouzian S, Bonomo RA, Good CE, Windau AR, Hujer AM, Occurrence, distribution, and origins of Streptococcus pneumoniae serotype 6C, a recently recognized serotype. J Clin Microbiol. 2009;47:64–72.
Suggested citation for this article: Choi EH, Lee HJ, Cho EY, Oh CE, Eun BW, Lee J, et al. Prevalence and genetic structures of Streptococcus pneumoniae serotype 6D, South Korea. Emerg Infect Dis [serial on the Internet]. 2010 Nov [date cited]. http://wwwnc.cdc.gov/eid/article/16/11/10-0941.htm
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