Emerging Infectious Disease ISSN: 1080-6059

Imported Leishmaniasis in Dogs, US Military Bases, Japan

Yuta Kawamura, Isao Yoshikawa, and Ken Katakura

Author affiliations: Author affiliations: Hokkaido University Graduate School of Veterinary Medicine, Sapporo, Hokkaido, Japan (Y. Kawamura, K. Katakura); US Army Japan District Veterinary Command–Zama Branch Kangawa, Japan (I. Yoshikawa)

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Figure

PCR amplification of the Leishmania spp.–specific small subunit rRNA gene from skin biopsy specimens from infected dogs, Japan. DNA samples (100–200 ng) were subjected to primary PCR (A), followed by nested PCR (B). Lanes 1–4, skin DNA samples from patient 1; lanes 5–9, skin DNA samples from patient 2; M, DNA molecular marker; P, positive control; N, negative control.

Figure. PCR amplification of the Leishmania spp.–specific small subunit rRNA gene from skin biopsy specimens from infected dogs, Japan. DNA samples (100–200 ng) were subjected to primary PCR (A), followed by nested PCR (B). Lanes 1–4, skin DNA samples from patient 1; lanes 5–9, skin DNA samples from patient 2; M, DNA molecular marker; P, positive control; N, negative control.

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