Volume 16, Number 12—December 2010
Dispatch
Mycobacterium tuberculosis Infection of Domesticated Asian Elephants, Thailand
Taweepoke Angkawanish, Worawidh Wajjwalku
, Anucha Sirimalaisuwan, Thattawan Kaewsakhorn, Kittikorn Boonsri, Victor P.M.G. Rutten, and SittidetMahasawangkul
, Anucha Sirimalaisuwan, Thattawan Kaewsakhorn, Kittikorn Boonsri, Victor P.M.G. Rutten, and SittidetMahasawangkulTable 1
Primers used to identify bacteria cultured from trunk wash and tissue samples from domesticated Asian elephants, Thailand, 2003–2008*
| Primer | Forward | Reverse |
|---|---|---|
| 16s rRNA | 5′-AgA gTT TgA TCC Tgg CTC Ag-3′ | 5′-ACg gCT ACC TTg TTA CgA CTT-3′ |
| ITS | 5′-TTg TAC ACA CCg CCg gTC a-3′ | 5′-TCT CgA TgC CAA ggC ATC CAC C-3′ |
| gyrB | 5′-TCG GAC GCG TAT GCG ATA TC-3′ | 5′-ACA TAC AGT TCG GAC TTG CG-3′ |
