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Volume 16, Number 2—February 2010

Letter

Neisseria meningitidis Serogroup W135, China

Suggested citation for this article

To the Editor: Neisseria meningitidis is a gram-negative bacterium found only in humans and is a major cause of serious invasive diseases. Before 2006, in the People’s Republic of China, all meningococcal diseases were caused by serogroups A, B, and C. However, there are >13 serogroups of this organism. Three cases of infection with N. meningitidis serogroup W135 were reported in China during 2006–2008. We describe these 3 meningitis patients and the N. meningitidis serogroup W135 strains isolated from these patients by genotyping methods.

Patient 1, a 36-year-old man, was seen at a local hospital in Fujian Province in January 2006. He became ill while on a business trip and was given a diagnosis by culture of an N. meningitidis infection. Patient 2, a 25-year-old man, was seen in Guangdong Province in May 2007. He had not traveled outside this area in the 10 days before becoming ill. Patient 3, a 14-year-old girl, was seen in Guangxi Province in February 2008. She was a middle school student and had toured the suburbs of this province with her classmates 2 days before becoming ill. Close contacts of all 3 patients were investigated; no additional N. meningitidis infections were detected. However, N. meningitidis was isolated from a throat swab specimen obtained from the younger cousin of patient 3.

N. meningitidis infection was confirmed for all 3 patients on the basis of clinical symptoms and laboratory results. All patients reported neck stiffness. Physical examinations showed Kernig signs, Brudzinski signs, and high temperatures (>38°C). Cerebrospinal fluid (CSF) samples were turbid with increased protein levels and pressure; leukocyte counts were increased (>5,000 cells/μL). CSF culture on chocolate agar grew N. meningitidis after 24 h. Isolates were identified as serogroup W135 by using specific antiserum (Remel, Lenexa, KS, USA) at provincial Centers for Disease Control and Prevention (CDC) in China and confirmed at the China CDC.

Patients were treated with antimicrobial drugs and recovered fully. An isolate from the cousin of patient 3 was also identified as W135. Etest strips and broth microdilution were used for antimicrobial drug susceptibility testing for the 4 W135 isolates. All isolates were susceptible to 12 antimicrobial drugs tested, which included therapeutic and prophylaxis agents used frequently in China.

Pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, and outer membrane protein (porA) gene variant region subtyping were used to characterize the 4 case-related W135 N. meningitidis isolates and other isolates from asymptomatic carriers. Strain R29057 (from France) was used as a reference strain. The 4 case-related isolates showed similar PFGE patterns. These patterns were distinct from those of other W135 isolates obtained from asymptomatic carriers. Three invasive disease isolates and 1 from the close contact of patient 3 had the same multilocus sequence type (ST) and PorA subtype; all were ST11: P1.5, 2. This subtype was not detected among other tested isolates of W135 obtained from asymptomatic carriers (Technical Appendix [PDF - 190 KB - 2 pages]).

ST11: P1.5, 2 N. meningitidis serogroup W135 was responsible for the epidemic of W135 meningococcal disease in 2000, which was associated with the Hajj pilgrimage in Saudi Arabia (1,2). The strain related to the Hajj pilgrimage was derived from clonal expansion within the ST11 complex/ET-37 complex (3). However, no epidemiologic data showed that the 3 cases in our study were linked to the Hajj pilgrimage. Since 2000, invasive diseases caused by W135 meningococci of ST11 have been reported in Africa, Asia, and the Middle East (4). ST11 W135 infections have been reported to cause invasive disease in Taiwan during 1996–2002 and were apparently introduced into Taiwan before the Hajj pilgrimage–associated outbreak because they were genotypically distinct from the Hajj-related W135 clone (5,6).

The 3 cases we report were observed in southeastern China near Taiwan (Technical Appendix [PDF - 190 KB - 2 pages]), but no direct epidemiologic links are known. Because of the lack of W135 strains from Hajj pilgrimages and Taiwan in this study, we could not provide a detailed and integrated genotypic relationship between the strains in China and those of Hajj pilgrimages and Taiwan. However, we can confirm that these 3 cases were caused by strains from the same hypervirulent clone characterized as ST11: P1.5, 2.

W135 strains have been isolated after vaccination with a bivalent meningococcal vaccine in Cameroon (7). In China, the bivalent meningococcal vaccine has been successfully introduced into the national expanded immunization program in response to an outbreak of N. meningitidis serogroup C during 2003–2004 (8).The 3 patients infected with W135 in our study did not receive bivalent meningococcal vaccines. W135 meningococcal disease appears to be an emerging problem that should be investigated epidemiologically. These patients highlight the need for further epidemiologic surveillance to monitor changes in the incidence of meningococcal disease caused by W135 and for improved public health disease control strategies in the future.

Zhujun ShaoComments to Author , Haijian Zhou, Yuan Gao, Hongyu Ren, Li Xu, Biao Kan, and Jianguo Xu
Author affiliations: Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China

Acknowledgments

We thank Leonard W. Mayer and Xin Wang for valuable comments and suggestions for preparing the manuscript.

This study was supported by grants from the Ministry of Health (200802016 and 2008ZX10004-008) and the Ministry of Science and Technology (2008ZX10004-008), People’s Republic of China.

References

  1. Aguilera JF, Perrocheau A, Meffre C, Hahne S. Outbreak of serogroup W135 meningococcal disease after the Hajj pilgrimage, Europe, 2000. Emerg Infect Dis. 2002;8:7617.PubMed
  2. Lingappa JR, Al-Rabeah AM, Hajjeh R, Mustafa T, Fatani A, Al-Bassam T, Serogroup W-135 meningococcal disease during the Hajj, 2000. Emerg Infect Dis. 2003;9:66571.PubMed
  3. Mayer LW, Reeves MW, Al-Hamdan N, Sacchi CT, Taha MK, Ajello GW, Outbreak of W135 meningococcal disease in 2000: not emergence of a new W135 strain but clonal expansion within the electrophoretic type-37 complex. J Infect Dis. 2002;185:1596605. DOIPubMed
  4. Apicella MA. Extrameningeal complications of Neisseria meningitidis serogroup W135 infection. Clin Infect Dis. 2004;38:16389. DOIPubMed
  5. Chiou CS, Liao JC, Liao TL, Li CC, Chou CY, Chang HL, Molecular epidemiology and emergence of worldwide epidemic clones of Neisseria meningitidis in Taiwan. BMC Infect Dis. 2006;6:25. DOIPubMed
  6. Yazdankhah SP, Lindstedt BA, Caugant DA. Use of variable-number tandem repeats to examine genetic diversity of Neisseria meningitidis. J Clin Microbiol. 2005;43:1699705. DOIPubMed
  7. Fonkoua MC, Taha MK, Nicolas P, Cunin P, Alonso JM, Bercion R, Recent increase in meningitis caused by Neisseria meningitidis serogroups A and W135, Yaoundé, Cameroon. Emerg Infect Dis. 2002;8:3279. DOIPubMed
  8. Shao Z, Li W, Ren J, Liang XF, Xu L, Diao BW, Identification of a new Neisseria meningitidis serogroup C clone from Anhui Province, China. Lancet. 2006;367:41923. DOIPubMed

Technical Appendix

Suggested citation for this article: Shao Z, Zhou H, Gao Y, Ren H, Xu L, Kan B, et al. Neisseria meningitidis serogroup W135, China [letter]. Emerg Infect Dis [serial on the Internet]. 2010 Feb [date cited]. http://wwwnc.cdc.gov/eid/article/16/2/09-0901

DOI: 10.3201/eid1602.090901

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Table of Contents – Volume 16, Number 2—February 2010

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Zhujun Shao, Department of Respiratory Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention. PO Box 5, Changping, Beijing 102206, People’s Republic of China

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