Volume 16, Number 2—February 2010
Dispatch
White-Nose Syndrome Fungus (Geomyces destructans) in Bat, France
Sébastien J. Puechmaille, Pascal Verdeyroux, Hubert Fuller, Meriadeg Ar Gouilh, Michaël Bekaert, and Emma C. Teeling
Table
Primers used for PCR amplification and sequencing of fungus in bats, France*
| Gene | Primer sequence (5′ → 3′) | PCR |
|---|---|---|
| ITS |
TCCTCCGCTTATTGATATGC | Forward |
| GGAAGTAAAAGTCGTAACAAGG |
Reverse |
|
| SSU rRNA | CTGGTTGATTCTGCCAGT | Forward |
| AAACCTTGTTACGACTTTTA | Reverse | |
| CCGGAGAAGGAGCCTGAGAAAC | † | |
| AACTTAAAGGAATTGACGGAAG | † | |
| CTCATTCCAATTACAAGACC | † | |
| GAGTTTCCCCGTGTTGAGTC | † |
*ITS, internal transcribed spacer; SSU, small subunit.
†Used for sequencing only.
