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Volume 16, Number 3—March 2010

Dispatch

Candidatus Bartonella mayotimonensis and Endocarditis

Eleanor Y. Lin1Comments to Author , Constantine Tsigrelis, Larry M. Baddour, Hubert Lepidi, Jean-Marc Rolain, Robin Patel, and Didier Raoult
Author affiliations: Mayo Clinic, Rochester, Minnesota, USA (E. Y. Lin, C. Tsigrelis, L.M. Baddour, R. Patel); Université de la Méditerranée, Marseille, France (H. Lepidi, J.M. Rolain, D. Raoult)

Main Article

Figure 1

A) Western blot of serum sample from patient infected with Candidatus Bartonella mayotimonensis. Left lane, Molecular mass standard; lane 1, Bartonella quintana; lane 2, B. henselae; lane 3, B. elizabethae; lane 4, B. vinsonii subsp. berkhoffii; lane 5, B. alsatica. Values on the left are in kilobases. B) Numerous darkly stained bacilli consistent with Bartonella species organized in clusters in the valvular vegetation (Warthin-Starry stain; original magnification ×400). C and D) Bacteria detect

Figure 1. A) Western blot of serum sample from patient infected with Candidatus Bartonella mayotimonensis. Left lane, Molecular mass standard; lane 1, Bartonella quintana; lane 2, B. henselae; lane 3, B. elizabethae; lane 4, B. vinsonii subsp. berkhoffii; lane 5, B. alsatica. Values on the left are in kilobases. B) Numerous darkly stained bacilli consistent with Bartonella species organized in clusters in the valvular vegetation (Warthin-Starry stain; original magnification ×400). C and D) Bacteria detected by immunohistochemical analysis of an extracellular location inside the valvular vegetation (polyclonal antibody against B. vinsonii subsp. berkhoffii, Warthin-Starry stain and hematoxylin counterstain; original magnification ×100 in C and ×400 in D).

Main Article

1Current affiliation: Massachusetts General Hospital, Boston, Massachusetts, USA

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