Figure 1. A) Western blot of serum sample from patient infected with Candidatus Bartonella mayotimonensis. Left lane, Molecular mass standard; lane 1, Bartonella quintana; lane 2, B. henselae; lane 3, B. elizabethae; lane 4, B. vinsonii subsp. berkhoffii; lane 5, B. alsatica. Values on the left are in kilobases. B) Numerous darkly stained bacilli consistent with Bartonella species organized in clusters in the valvular vegetation (Warthin-Starry stain; original magnification ×400). C and D) Bacteria detected by immunohistochemical analysis of an extracellular location inside the valvular vegetation (polyclonal antibody against B. vinsonii subsp. berkhoffii, Warthin-Starry stain and hematoxylin counterstain; original magnification ×100 in C and ×400 in D).