Jozsef Szelei, Kaiyu Liu, Yi Li, Sandra Fernandes, and Peter Tijssen
Author affiliations: Institut National de la Recherche Scientifique–Institut Armand-Frappier, Laval, Quebec, Canada (J. Szelei, K. Liu, Yi Li, S. Fernandes, P. Tijssen); Central People’s Republic of China Normal University, Wuhan, People’s Republic of China (Y. Li)
Figure 1. Parallel PCR amplification of PARV4-like (A) and PPV (B) by using purified DNA from clotting FVIII preparations. The results of this PCR usually suggested a higher PARV4 load despite the higher efficiency of the PPV PCR (J. Szelei and P. Tijssen, unpub. data). This finding was confirmed with the quantitative MIMIC PCR method for PPV (11). Numbers indicate different lots of FVIII prepared in 1:1994A, 2:1994B, 3:1996A, 4:1996B, 5:1999, 6:2000A, 7:2000B, 8:2001A, 9:2001B, 10:2001C, 11:2001D, and 12: DNA marker (1-kb ladder; Invitrogen, Carlsbad, CA USA). PARV4, parvovirus 4; PPV, porcine parvovirus; FVIII, factor VIII.
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