Volume 16, Number 3—March 2010
Research
Blood Meal Analysis to Identify Reservoir Hosts for Amblyomma americanum Ticks
Brian F. Allan
, Lisa S. Goessling, Gregory A. Storch, and Robert E. Thach
, Lisa S. Goessling, Gregory A. Storch, and Robert E. ThachTable 1
Oligonucleotide sequences of bacterial probes used in reverse line blot assay
| Probe ID | Nucleotide sequence (5′ → 3′) | Target organism (RNA genes) | Reference sequence |
|---|---|---|---|
| Ptg011 | aacatgaacatctaaaaacataaa | Borrelia garinii (23S–5S) | * |
| Ptg012 | aacatttaaaaaataaattcaagg | B. afzelii (23S–5S) | * |
| Ptg013 | cattaaaaaaatataaaaaataaatttaagg | B. valaisiana (23S–5S) | * |
| Ptg009 | ctttgaccatatttttatcttcca | B. burgdorferi s.l. (23S––5S) | * |
| Ptg010 | aacaccaatatttaaaaaacataa | B. burgdorferi s.s. (23S–5S) | * |
| Ptg003 | cgaacttctgggtcaagac | B. burgdorferi s.l. (16S) | † |
| Ptg020 | agataactactctccgtttg | B. lonestari (16S) | AY166715 |
| Ptg022 | tcctaatagggggagtc | Ehrlichia chaffeensis (16S) | M73222 |
| Ptg023 | cttttaacagagggagtca | E. ewingii (16S) | M73227 |
| Ptg024 | tcctaacagggggagtc | E. canis/ovina/muris (16S) | AY394465, AY318946, ABO13009 |
| Ptg007 | tggggattttttatctctgtg | Anaplasma phagocytophilum (16S) | † |
| Ptg021 | ctaccactgacgctgat | Rickettsia rickettsii (16S) | DQ150694 |
| Ptg027 | cttcggaacgcagtgac | Francisella tularensis + F. philomiragia (16S) | Z21932, Z21933 |
| Ptg026 | cttggggaggacgttac | F. tularensis subsp. tularensis (16S) | Z21932 |
| Ptg029 | gcctatragttaatagcttgt | F. philomiragia (16S) | Z21933 |
| Ptg028 | tcctgcgatctttctaga | F. endosymbiont of Dv (16S) | AF166256 |
| Ptg032 | catccagggaagtaagc | Arsenophonus spp. (16S) | AY265347 |
| Ptg030 | gctacaactgacactgatg | R. endosymbiont of Dv (16S) | AY375427 |
| Ptg031 | tacaactgacgctaatgc | R. amblyommii + Rickettsia sp. (16S) | U11012 |
| Ptg035 | tcggaagattatctttcgg | R. amblyommii (16S) | U11012 |
