Volume 16, Number 3—March 2010
Research
Blood Meal Analysis to Identify Reservoir Hosts for Amblyomma americanum Ticks
Brian F. Allan
, Lisa S. Goessling, Gregory A. Storch, and Robert E. Thach
, Lisa S. Goessling, Gregory A. Storch, and Robert E. ThachTable 2
Oligonucleotide sequences of vertebrate probes used in reverse line blot assay
| Probe ID | Probe name | Nucleotide sequence (5′ → 3′) | Reference sequence |
|---|---|---|---|
| PRNA010 | Aves | ccgacctccggggacgc | * |
| PRNA012 | Passeriformes | gggcccgcccggcagct | * |
| PRNA029 | Galliformes | gggctcgcccggcggct | * |
| PRNA042 | Squamata/testudines | cgctgacctccggggatgc | Sceloporus undulatus (M36359, M59400), Crotaphytus collaris collaris† (FJ797666), Trachemys scripta† (FJ797668), Scincella lateralis (AY217908), Eumeces fasciatus (AY217920), Elaphe obsoleta† (FJ797667), Heterodon platirhinos (M59392) |
| PRNA043 | Amphibia | cgctgacccccagggatgc | Rana amurensis (AF542043), R. chensinensis (AY145522), Xenopus laevis (X04025) |
| PRNA045 | Ruminantia | ggtcagcctcctcccggc | Odocoileus virginianus† (FJ797665), Capreolus capreolus L (AY150545), Cervus elaphus L (AY150547), Bos taurus (AY779625) |
| PRNA018 | Leporidae | cgggggggtgggcgccg | * |
| PRNA047 | Leporidae/carnivore | ggtcagcctccccccggc | Sylvilagus floridanus (FJ797663), Procyon lotor† (FJ797659), Felis catus L (AY150542), |
| PRNA046 | Canidae | ggtcagcctccctccggc | Canis latrans† (FJ797662), Canis lupus familiaris† (FJ797658), C. lupus familiaris (DQ287955), Vulpes vulpes (AY150549) |
| PRNA026 | Sciurus | cggtcagcttccccccgg | * |
| PRNA037 | Blarina | agcctcccctcggctccg | Blarina sp.† (FJ797661) |
| PRNA030 | Erinaceus | ctccctccggctccggc | * |
| PRNA017 | Myodes 1 | gagctcccccgcggccc | * |
| PRNA050 | Myodes 2 | cgacgggcgccgacccc | Myodes glareolus (AY150543) |
| PRNA011 | Murinae/gerbilinae | ccctcccggctccggccg | * |
| PRNA034 | Rattus | cggtcagccccctcccgg | Rattus norvegicus (X01117) |
| PRNA033 | Mus | ccggtgagctccctcccgg | Mus musculus (X00686) |
| PRNA035 | Sigmodontinae | tcagctccctcccggcccc | Peromyscus sp.† (FJ797660), Peromyscus leucopus (AY591913) |
| PRNA032 | Didelphis | cggcggcttccccctaacc | Didelphis virginiana (J311677) |
| PRNA048 | Mephitis | ggtcagcctctccccggc | Mephitis mephitis† (FJ797664) |
*Designed by Pichon et al. 2003 (13).
†Sequence obtained in this study.
