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Volume 16, Number 3—March 2010

Research

Blood Meal Analysis to Identify Reservoir Hosts for Amblyomma americanum Ticks

Brian F. AllanComments to Author , Lisa S. Goessling, Gregory A. Storch, and Robert E. Thach
Author affiliations: Washington University in St. Louis, St. Louis, Missouri, USA (B.F. Allan, L.S. Goessling, R.E. Thach); St. Louis Children’s Hospital, St. Louis (G.A. Storch)

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Table 3

Hybridization by host DNA to vertebrate reverse line blot probes

Probe ID Probe name Vertebrate DNA hybridized
PRNA010 Aves Turdus migratorius, Meleagris gallopavo, Gallus gallus, Chen caerulescens
PRNA012 Passeriformes T. migratorius
PRNA029 Galliformes M. gallopavo, G. gallus, C. caerulescens
PRNA042 Squamata/testudines Crotophytus collaris, Elaphe obsoleta, Trachemys scripta elegans
PRNA043 Amphibia Rana clamitans
PRNA045 Ruminantia Odocoileus virginianus, Cervus elephus, Bos taurus, Sus scrofa domestica
PRNA018 Leporidae Sylvilagus floridanus, Sus scrofa domestica
PRNA047 Leporidae/carnivora S. floridanus, Felis catus, Procyon lotor
PRNA046 Canidae Canis lupus familiaris, C. latrans, Vulpes vulpes, Tamias striatus*
PRNA026 Sciurus Sciurus carolinensis, Sciurus niger, S. griseus, Marmota monax
PRNA037 Blarina Blarina brevicauda, Sorex vagrans
PRNA030 Erinaceus No hybridization with any vertebrate DNA tested
PRNA017 Myodes 1 Myodes gapperi
PRNA050 Myodes 2 M. gapperi, Microtus californicus
PRNA011 Murinae/gerbilinae Rattus norvegicus, Mus musculus, Zapus hudsonius
PRNA034 Rattus Rattus norvegicus
PRNA033 Mus M. musculus
PRNA035 Sigmodontinae Peromyscus spp., Neotoma fuscipes
PRNA032 Didelphis Didelphis virginiana
PRNA048 Mephitis Mephitis mephitis

*The reaction was confirmed by using 2 tissue samples. The PCR amplicon was sequenced and matches the Canidae probe.

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