Emerging Infectious Disease ISSN: 1080-6059

Transmission of West Nile Virus during Horse Autopsy

Marietjie Venter

, Johan Steyl, Stacey Human, Jacqueline Weyer, Dewald Zaayman, Lufcille Blumberg, Patricia A. Leman, Janusz Paweska, and Robert Swanepoel

Author affiliations: University of Pretoria, Pretoria, South Africa (M. Venter, J. Steyl, S. Human, D. Zaayman); National Institute for Communicable Diseases, Sandringham, South Africa (M. Venter, J. Weyer, L. Blumberg, P.A. Lehman, J. Paweska, R. Swanepoel)

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Figure

Phylogenetic comparison of West Nile virus (WNV) nonstructural protein 5 partial gene fragment identified in a veterinary student’s serum and in the virus isolate obtained from mouse brain and the horse’s brain after autopsy (triangles) relative to other WNV strains from South Africa and elsewhere. The neighbor-joining tree was compiled by using MEGA version 4 software (www.megasoftware.net/under) and 1,000 bootstrap replicates by using the maximum composite likelihood algorithm. Genetic lineage

Figure. Phylogenetic comparison of West Nile virus (WNV) nonstructural protein 5 partial gene fragment identified in a veterinary student’s serum and in the virus isolate obtained from mouse brain and the horse’s brain after autopsy (triangles) relative to other WNV strains from South Africa and elsewhere. The neighbor-joining tree was compiled by using MEGA version 4 software (www.megasoftware.net/under) and 1,000 bootstrap replicates by using the maximum composite likelihood algorithm. Genetic lineages are indicated on the right as described (3–7). Scale bar indicates nucleotide substitutions per site. JEV, Japanese encephalitis virus (included as outgroup).

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