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Volume 16, Number 3—March 2010

Letter

Human Herpesvirus 8, Southern Siberia

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Technical Appendicies

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To the Editor: Human herpesvirus 8 (HHV-8) is the etiologic agent of Kaposi sarcoma. Sequence analysis of the highly variable open reading frame (ORF)–K1 of HHV-8 has enabled the identification of 5 main molecular subtypes, A–E (1). A and C subtypes are prevalent in persons in Europe, Mediterranean countries, northwestern China, and the United States; subtype B, in persons in sub-Saharan Africa; subtype D, in persons in the Pacific Islands and Japan (26); and subtype E, in Native Americans in the United States.

Considering that K1 gene polymorphisms of HHV-8–infected persons reflect the divergence accumulated during the early migrations of modern humans out of Africa (1), it is tempting to put the polymorphisms observed in the different subtypes into an evolutionary perspective with their geographic distribution. It is thought that Native Americans infected by subtype E and Pacific Islanders, including those infected by subtype D in the Japanese archipelago, originated from a common ancestral genetic stock in continental Asia. Because Siberia constitutes the geographic link between mainland Asia, North America, and the Pacific (Technical Appendix [PDF - 811 KB - 4 pages]), it is likely that the Siberian region has served as a source or a corridor of human dispersals to these regions. Thus, we conducted a molecular epidemiology HHV-8 survey of the Buryat population, a major indigenous group in southern Siberia, to gain new insights into the origins, possibly common, of HHV-8 subtypes D and E.

Figure

Thumbnail of Age-dependent human herpesvirus 8 (HHV-8) seroprevalence rates for 745 persons in southern Siberia 25–98 years of age who lived in the Ust Orda, Ulan Ude, or Chita districts during 1995. Seropositivity was based on strict criteria; only samples showing punctuate nuclear staining clearly reactive at a dilution >1:160 were considered HHV-8 positive. All 187 HHV-8–seropositive samples were tested for antibodies directed against HIV-1/2 by using Genscreen HIV-1/2 Antibody Assay (Bio-

Figure. Age-dependent human herpesvirus 8 (HHV-8) seroprevalence rates for 745 persons in southern Siberia 25–98 years of age who lived in the Ust Orda, Ulan Ude, or Chita districts during 1995. Seropositivity...

After consent of local authorities and participants, we collected 745 human blood samples in 1995 in 17 medicosocial structures (homes for elderly persons, veterans of the Russian army, hospitalized persons, blood donors) located near Lake Baïkal and originating from Ulan Ude (344), Ust Orda (216), and Chita (185), Siberia, Russia (additional data can be obtained directly from the authors). The median age of those included was 52 years (range 25–98 years); 489 (66%) were women. Antibodies against HHV-8 latency–associated nuclear antigen were identified by immunofluorescent antibody assay by using the BC3 cell line (3). Punctuate nuclear staining of BC3 cells at a 1:160 dilution was observed for 187 (25.1%) patients with no difference according to investigated regions (p = 0.32 by χ2 test) or between men (25.8%) and women (24.7%) (p = 0.76 by χ2 test; Technical Appendix [PDF - 811 KB - 4 pages]). However, HHV-8 seroprevalence increased with patient age, rising from 12.9% (25–43 years) to 46.4% (>61 years) (p = 1.8 × 10–13 by χ2 test for trend) (Figure; Technical Appendix [PDF - 811 KB - 4 pages]). No significant difference was observed in antibody titers according to age (p = 0.45 by Fisher exact test). These results demonstrate that HHV-8 infection is highly prevalent in the Siberian adult population tested.

HHV-8 infection was determined by nested PCR that amplified a 737-bp fragment of the ORFK1 in peripheral blood buffy coats of 85 HHV-8–seropositive and 10 HHV-8–seronegative persons (3). Amplification was positive in 19/85 (22.4%) samples; sequences were obtained for 18 of these samples (Technical Appendix [PDF - 811 KB - 4 pages]). These sequences showed 0%–7.31% nucleotide divergence and 0%–3.55% amino acid divergence. Nevertheless, 17 strains were found to be closely related with <1.75% nucleotide differences for 684 nt, and only 1 sequence (1445 strain) displayed higher nucleotide divergence.

A comparative sequence analysis, including 66 representatives of K1 gene sequences of the HHV-8 A/C subtypes/subgroups, and sequences obtained from persons originating from Russia, was performed (79). Seventeen of the 18 HHV-8 strains from Siberia belonged to the A subtype; 15 clustered in a newly identified specific subclade (Technical Appendix [PDF - 811 KB - 4 pages]). Notably, the 1445-Siberian strain, which exhibits the typical 5 aa deletion at positions 201–205, belongs to subtype C and clustered with the 7848 strain previously described by Lacoste et al. (9). Furthermore, both strains originate from Chita.

Our results indicate that HHV-8 infection is highly prevalent in the population tested in southern Siberia and extend current knowledge on the worldwide distribution of HHV-8 genotypes. The presence of a Siberian strain monophyletic subclade suggests the existence of HHV-8 strains preferentially spreading among this population in southern Siberia.

To ascertain the maternal ancestry of these persons, we sequenced the hypervariable region I (HVS-I) of the maternally-inherited mitochondrial DNA (mtDNA) and assigned haplogroups on the basis of the HVS-I motifs. Our analyses showed that 17/18 persons analyzed showed a mtDNA motif of clear continental east Asian origin (e.g., A, D correspond to different mtDNA haplogroups). One person (1474-strain) had a lineage (i.e., HV1) that is thought to have a western Eurasian origin. Overall, these mtDNA analyses indicate that the maternal ancestry of the persons examined here can be unambiguously attributed to East Asia, and not to Western Eurasia. K1 subtype A sequences recently found in the Xinjiang Uygur region in China (10) do not correspond to the specific Siberian clade described in our study. Thus, we must now consider that the widely distributed HHV-8 A/C subtype, so far mainly observed in Europe and Mediterranean countries, is also largely predominant in continental Asia.

Olivier Cassar, Sylviane Bassot, Sabine Plancoulaine, Lluis Quintana-Murci, Christine Harmant, Vladimir Gurtsevitch, Natalia B. Senyuta, Larissa S. Yakovleva, Guy de Thé, and Antoine GessainComments to Author 
Author affiliations: Institut Pasteur, Paris, France (O. Cassar, S. Bassot, S. Plancoulaine, L. Quintana-Murci, C. Harmant, G. de Thé, A. Gessain); Institut National de la Santé et de la Recherche Médicale (INSERM), Paris (S. Plancoulaine); Centre National de la Recherche Scientifique (CNRS), Paris (L. Quintana-Murci); Blokhin Cancer Research Center, Moscow, Russia (V. Gurtsevitch, N.B. Senyuta, L.S. Yakovleva)

References

  1. Hayward GS, Zong JC. Modern evolutionary history of the human KSHV genome. Curr Top Microbiol Immunol. 2007;312:142. DOIPubMed
  2. Duprez R, Cassar O, Hbid O, Rougier Y, Morisse L, Bassot S, Cutaneous disseminated endemic Kaposi’s sarcoma in a Polynesian man infected with a new divergent human herpesvirus 8 subtype D. J Clin Virol. 2006;37:2226. DOIPubMed
  3. Cassar O, Afonso PV, Bassot S, Plancoulaine S, Duprez R, Capuano C, Novel human herpesvirus 8 subtype D strains in Vanuatu, Melanesia. Emerg Infect Dis. 2007;13:17458.PubMed
  4. Zong JC, Ciufo DM, Alcendor DJ, Wan X, Nicholas J, Browning PJ, High-level variability in the ORF-K1 membrane protein gene at the left end of the Kaposi’s sarcoma–associated herpesvirus genome defines four major virus subtypes and multiple variants or clades in different human populations. J Virol. 1999;73:415670.PubMed
  5. Meng YX, Spira TJ, Bhat GJ, Birch CJ, Druce JD, Edlin BR, Individuals from North America, Australasia, and Africa are infected with four different genotypes of human herpesvirus 8. Virology. 1999;261:10619. DOIPubMed
  6. Cook PM, Whitby D, Calabro ML, Luppi M, Kakoola DN, Hjalgrim H, Variability and evolution of Kaposi’s sarcoma–associated herpesvirus in Europe and Africa. International Collaborative Group. AIDS. 1999;13:116576. DOIPubMed
  7. Davidovici B, Karakis I, Bourboulia D, Ariad S, Zong J, Benharroch D, Seroepidemiology and molecular epidemiology of Kaposi’s sarcoma–associated herpesvirus among Jewish population groups in Israel. J Natl Cancer Inst. 2001;93:194202. DOIPubMed
  8. Kadyrova E, Lacoste V, Duprez R, Pozharissky K, Molochkov V, Huerre M, Molecular epidemiology of Kaposi’s sarcoma–associated herpesvirus/human herpesvirus 8 strains from Russian patients with classic, posttransplant, and AIDS-associated Kaposi's sarcoma. J Med Virol. 2003;71:54856. DOIPubMed
  9. Lacoste V, Kadyrova E, Chistiakova I, Gurtsevitch V, Judde JG, Gessain A. Molecular characterization of Kaposi’s sarcoma–associated herpesvirus/human herpesvirus-8 strains from Russia. J Gen Virol. 2000;81:121722.PubMed
  10. Fu B, Sun F, Li B, Yang L, Zeng Y, Sun X, Seroprevalence of Kaposi’s sarcoma–associated herpesvirus and risk factors in Xinjiang, China. J Med Virol. 2009;81:142231. DOIPubMed

Figure

Technical Appendix

Suggested citation for this article: Cassar O, Bassot S, Plancoulaine S, Quintana-Murci L, Harmant C, Gurtsevitch V, et al. Human herpesvirus 8, southern Siberia [letter]. Emerg Infect Dis [serial on the Internet]. 2010 Mar [date cited]. http://wwwnc.cdc.gov/eid/article/16/3/09-1390

DOI: 10.3201/eid1603.091390

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Table of Contents – Volume 16, Number 3—March 2010

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Antoine Gessain, Unité d’Epidémiologie et Physiopathologie des Virus Oncogènes – Unité de Recherche Appliquée, 3015, Département de Virologie, Bâtiment Lwoff, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris CEDEX 15, France

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