Contribution of Streptococcus anginosus to Infections Caused by Groups C and G Streptococci, Southern India
Silvana Reißmann, Claudia Friedrichs, Reena Rajkumari, Andreas Itzek, Marcus Fulde, Arne C. Rodloff, Kootallur N. Brahmadathan, Gursharan S. Chhatwal, and D. Patric Nitsche-Schmitz
Author affiliations: Helmholtz Centre for Infection Research, Braunschweig, Germany (S. Reißmann, A. Itzek, M. Fulde, G.S. Chhatwal, D.P. Nitsche-Schmitz); University Hospital Leipzig, Leipzig, Germany (C. Friedrichs, A.C. Rodloff); Christian Medical College, Vellore, Tamil Nadu, India (R. Rajkumari, K.N. Brahmadathan)
Figure. Amplification and characterization of a newly discovered open reading frame (ORF) of Streptococcus anginosus. A) Gel electrophoresis after emm-PCR on S. anginosus isolate SV52 (SV52) and S. pyogenes strain A60 (A60). The latter isolate was used as a control that possesses an emm3 gene. The S. anginosus strain generated a low concentration 1.1-kb amplicon, as compared with the 1.4-kb product of the S. pyogenes strain. Inverse PCR based on the 1.1-kb sequence of SV52 showed an ORF of 3,363 bp. Its sequence is predicted to code for the membrane protein that is schematically depicted in panel B. It comprises an N terminal signal peptide (S) followed by a large extracellular region of 60 kDa (ECR1), 7 transmembrane helixes (1–7), and another large extracellular region of 23 kDa situated between the fourth and the fifth transmembrane helix (ECR3). Positions of the 2 alternative marker of Streptococcus anginosus and S. constellatus PCR products (I and II) relative to the full-length sequence and their length are indicated in basepairs.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.