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Volume 16, Number 4—April 2010

Research

Contribution of Streptococcus anginosus to Infections Caused by Groups C and G Streptococci, Southern India

Silvana Reißmann, Claudia Friedrichs, Reena Rajkumari, Andreas Itzek, Marcus Fulde, Arne C. Rodloff, Kootallur N. Brahmadathan, Gursharan S. Chhatwal, and D. Patric Nitsche-SchmitzComments to Author 
Author affiliations: Helmholtz Centre for Infection Research, Braunschweig, Germany (S. Reißmann, A. Itzek, M. Fulde, G.S. Chhatwal, D.P. Nitsche-Schmitz); University Hospital Leipzig, Leipzig, Germany (C. Friedrichs, A.C. Rodloff); Christian Medical College, Vellore, Tamil Nadu, India (R. Rajkumari, K.N. Brahmadathan)

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Figure

Amplification and characterization of a newly discovered open reading frame (ORF) of Streptococcus anginosus. A) Gel electrophoresis after emm-PCR on S. anginosus isolate SV52 (SV52) and S. pyogenes strain A60 (A60). The latter isolate was used as a control that possesses an emm3 gene. The S. anginosus strain generated a low concentration 1.1-kb amplicon, as compared with the 1.4-kb product of the S. pyogenes strain. Inverse PCR based on the 1.1-kb sequence of SV52 showed an ORF of 3,363 bp. Its

Figure. Amplification and characterization of a newly discovered open reading frame (ORF) of Streptococcus anginosus. A) Gel electrophoresis after emm-PCR on S. anginosus isolate SV52 (SV52) and S. pyogenes strain A60 (A60). The latter isolate was used as a control that possesses an emm3 gene. The S. anginosus strain generated a low concentration 1.1-kb amplicon, as compared with the 1.4-kb product of the S. pyogenes strain. Inverse PCR based on the 1.1-kb sequence of SV52 showed an ORF of 3,363 bp. Its sequence is predicted to code for the membrane protein that is schematically depicted in panel B. It comprises an N terminal signal peptide (S) followed by a large extracellular region of 60 kDa (ECR1), 7 transmembrane helixes (1–7), and another large extracellular region of 23 kDa situated between the fourth and the fifth transmembrane helix (ECR3). Positions of the 2 alternative marker of Streptococcus anginosus and S. constellatus PCR products (I and II) relative to the full-length sequence and their length are indicated in basepairs.

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