Volume 16, Number 4—April 2010
Research
Contribution of Streptococcus anginosus to Infections Caused by Groups C and G Streptococci, Southern India
Silvana Reißmann, Claudia Friedrichs, Reena Rajkumari, Andreas Itzek, Marcus Fulde, Arne C. Rodloff, Kootallur N. Brahmadathan, Gursharan S. Chhatwal, and D. Patric Nitsche-Schmitz
Table 1
Sequences of primers used in study of groups C and G streptococci, Vellore, India, and Leipzig, Germany*
| Name | Sequence (5′ → 3′) | Application |
|---|---|---|
| 16S rDNA fwd | AGAGTTTGATCCTGGCTC | 16S rDNA amplification |
| 16S rDNA rev |
GGTTACCTTGTTACGACTT |
|
| Primer 1 | TATTCGCTTAGAAAATTAA | emm amplification |
| Primer 2 |
GCAAGTTCTTCAGCTTGTTT |
|
| M13 rev | CAATTTCACACAGGAAACAGCTATGAC | Sequencing of 1.1-kb fragment of moac |
| M13 fwd |
GTAAAACGACGGCCAGTGAATTG |
|
| moac1 | CAAGGAATTGATTCAGCAACAGTGC | Inverse PCR and sequencing of moac |
| moac3 | CTTCTCAACAAGCATTGGCAGATGC | |
| moac6 | GTGTGTATACACGTCGGACATTTCC | |
| moac7 | GGTACAGTAATGGGAAGTTTGTTAGG | |
| moac8 | GCGGATTGACTTCATTTGGCGTCG | |
| moac9 | GGTTTGGGGATGTCTTCTTCCATGG | |
| moac10 | GCATCTCAAATCAGACGAGCAAGC | |
| moac11 | CTTGAACTTGTCTTCGCATGGAGC | |
| moac12 | GACTATTATCAAACGGTATTTGCTCG | |
| moac2 | CCAATTCACTTGAATTGACGAATCC | |
| moac4 | GCCCAACCTGAAGACAGTTGAGC | |
| moac5 | CTGACGAAAAGAGAGCCAGATATCC | |
| moac13 | CTGATACCATAATCTGACATCACTGC | |
| moac14 |
GAAGTTGAACTATCTCCAATCACCG |
|
| moac-SP | ATGAAAAAATCCATTCTAAATAAGGATATC | Screening for 3,272-bp fragment of moac |
| moac-TMH7 |
AAGACTGGCACAAGATATAC |
|
| moac-BamH1 | GCGGATCCGGTCATTTTCCAAGCAAGG | Screening for 962-bp fragment of moac |
| moac-Sal1 | GCTGTCGACTTATTAAATTCAGCCTGCTTTTTCTCC |
*moac, marker of Streptococcus anginosus and S. constellatus.
