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Volume 16, Number 5—May 2010

Letter

Fluoroquinolone-Resistant Typhoid, South Africa

Suggested citation for this article

To the Editor: Salmonella enterica serotype Typhi, the causal pathogen for typhoid, is a major public health hazard in many parts of the world, with an estimated 21.6 million cases of typhoid and 217,000 deaths occurring each year (1). Most isolates in South Africa are susceptible to quinolones, and fluoroquinolones remain the treatment of choice (2). The disease is primarily water or foodborne, but person-to-person spread is well recognized (3). Travelers to disease-endemic regions may be at risk for typhoid, which may result in the importation of strains of S. Typhi with unfamiliar or unusual resistance patterns (4). Such infections present a challenge to local clinicians on optimal patient management.

S. Typhi was isolated from the blood culture of a woman 65 years of age from Cape Town; she had been in contact with a traveler to Bangladesh. The patient was treated first with ciprofloxacin, but this medication was changed to high-dose ceftriaxone combined with doxycycline for 8 days; she recovered well. Contact tracing indicated no family members had typhoid fever or carried the organism. The person who had traveled to Bangladesh was unavailable to provide further history or a stool specimen. No other potential source of infection could be elucidated: the patient lived in an urban area with safe water sources and shared meals with her family.

The isolate was referred to the Enteric Diseases Reference Unit for confirmation of identification, serotyping (Kauffman-White scheme), and antimicrobial drug susceptibility testing using the Etest (bioMérieux, Marcy l’Étoile, France) and agar dilution methods, according to criteria of the Clinical and Laboratory Standards Institute (Wayne, PA, USA) (www.clsi.org). The isolate was resistant to ampicillin, chloramphenicol, sulfamethoxazole, nalidixic acid, and ciprofloxacin, but susceptible to ceftriaxone and tetracycline.

Figure

Thumbnail of Dendrogram of pulsed-field gel electrophoresis patterns representative of the 15 largest clusters of Salmonella enterica serotype Typhi isolates identified in South Africa during 2005–2009. The pattern of isolate 372460 is indicated. Scale bar represents percentage similarity of pathogens.

Figure. Dendrogram of pulsed-field gel electrophoresis patterns representative of the 15 largest clusters of Salmonella enterica serotype Typhi isolates identified in South Africa during 2005–2009. The pattern of isolate 372460 is indicated....

Pulsed-field gel electrophoresis (PFGE) analysis was performed on the isolate, following the standard PulseNet protocol (5). The PFGE pattern was compared with a database of S. Typhi PFGE patterns from South Africa by using BioNumerics version 6.01 software (Applied Maths, Sint-Martens-Latem, Belgium). The PFGE pattern of this isolate was 100% identical to pattern JPPX01.0026 in the Global PulseNet Salmonella Typhi Database. This pattern, which has been reported to PulseNet from India, Kenya, Tanzania, and Taiwan, is the most common pattern in the database (www.pulsenetinternational.org/projects/styphidatabase.asp); it is rarely seen in South Africa (Figure).

PCR was used to isolate the quinolone resistance-determining region (QRDR) of gyrA, gyrB, parC, and parE (6). Genes were sequenced by using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and an Applied Biosystems 3130 genetic analyzer. The QRDR DNA sequences were compared with those of S. Typhi strain Ty2 (GenBank accession no. AE014613). PCR also used to confirm the presence of qnrA, qnrB, and qnrS genes (6). Analysis for mutations in the QRDR of gyrA, gyrB, parC, and par found a single amino-acid mutation (Ser83 to Tyr) in gyrA. No amino acid mutations were identified in gyrB, parC, and parE. PCR for detection of qnr genes confirmed the presence of a qnrS gene, which was identified as the qnrS1 variant by nucleotide sequence analysis.

The efflux of quinolones from bacterial cells was investigated in the following manner. For nalidixic acid and ciprofloxacin, agar dilution MIC testing was performed in the absence and presence of 40 µg/mL of the efflux pump inhibitor, Phe-Arg-β-naphthylamide (6). In the presence of efflux pump inhibitor, the MIC to ciprofloxacin decreased from 4 µg/mL to 1 µg/mL, and the MIC to nalidixic acid decreased from >512 µg/mL to 32 µg/mL, establishing the involvement of an efflux pump in conferring quinolone resistance.

The mutation in gyrA (Ser83 to Tyr) confers reduced susceptibility to ciprofloxacin to a maximum MIC ≈0.5 µg/mL (7) and the QnrS1 protein confers reduced susceptibility to ciprofloxacin to a maximum MIC ≈0.5 µg/mL (8). We showed that a single amino acid mutation in gyrA (Ser83 to Tyr) with the QnrS1 protein and active efflux, conferred ciprofloxacin resistance, at least to an MIC level of 4 µg/mL. Previously, Smith et al. reported quinolone resistance in South African isolates of S. Typhi mediated by mutations in gyrA and parC in combination with active efflux (6). We report qnrS1 from S. Typhi, confirming the role of plasmid-mediated fluoroquinolone resistance in S. Typhi (9) and a fluoroquinolone- resistant strain in South Africa. Fluoroquinolone resistance is well recognized in Bangladesh; other researchers have described multidrug-resistant S. Typhi isolates imported from that country (10). Molecular epidemiology supports the conclusion that this strain likely originated in Bangladesh (L. Theobald, pers.comm.).

In conclusion, fluoroquinolone-resistant typhoid fever is a reality in South Africa in patients who have a history of travel or contact with travelers. Blood cultures are mandatory to guide antimicrobial drug management. Plasmid-mediated fluoroquinolone resistance has implications for cotransference of resistance to the major antimicrobial agents used to treat typhoid fever and for the potential for rapid spread of fluoroquinolone resistance through S. Typhi strains in South Africa. The presence of fluoroquinolone-resistant typhoid fever could force a change in current treatment guidelines for this disease.

Karen H. KeddyComments to Author , Anthony M. Smith, Arvinda Sooka, Husna Ismail, and Stephen Oliver
Author affiliations: National Institute of Communicable Diseases, Johannesburg, South Africa (K.H. Keddy, A.M. Smith, A. Sooka, H. Ismail); University of the Witwatersrand, Johannesburg (K.H. Keddy, A.M. Smith, H. Ismail); National Health Laboratory Service, Groote Schuur, Cape Town, South Africa (S. Oliver); University of Cape Town, Cape Town (S. Oliver)

Acknowledgments

We thank Lisa Theobald and the Centers for Disease Control and Prevention, Atlanta, GA, USA, for information on comparative PFGE patterns in the Global PulseNet Salmonella Typhi Database. We also thank George Jacoby for providing control strains for qnr PCR and Malcolm Cupido for providing information on tracing of the patient and of the patient’s contacts.

The following institutions have contributed PFGE patterns to the Global PulseNet Salmonella Typhi Database, with which our strain was compared: National Institute of Cholera and Enteric Diseases, Kolkata, India; Central Laboratories, Ministry of Health, Jerusalem, Israel; Taiwan Centers for Disease Control, Taipei, Taiwan; Research Institute for Tropical Medicine, Department of Health, Manila, the Philippines; China Center for Disease Control and Prevention, Beijing, China; Duke University–Kilimanjaro Christian Medical Centre Collaboration, Moshi, Tanzania; University College Hospital, Galway, Ireland. This study was undertaken as part of the mandated responsibility of the Enteric Diseases Reference Unit of the National Institute for Communicable Diseases.

References

  1. Crump JA, Luby SP, Mintz ED. The global burden of typhoid fever. Bull World Health Organ. 2004;82:34653.PubMed
  2. Group for Enteric Respiratory and Meningeal Disease Surveillance in South Africa. GERMS-SA Annual Report 2008. 2009 [cited 2010 Mar 9]. http://www.nicd.ac.za/units/germs/annual/germssa_ann_report_2008.pdf
  3. World Health Organization. Background document: the diagnosis, treatment and prevention of typhoid fever. Geneva: The Organization, 2003.
  4. Kubota K, Barrett TJ, Ackers ML, Brachman PS, Mintz ED. Analysis of Salmonella enterica serotype Typhi pulsed-field gel electrophoresis patterns associated with international travel. J Clin Microbiol. 2005;43:12059. DOIPubMed
  5. Ribot EM, Fair MA, Gautom R, Cameron DN, Hunter SB, Swaminathan B, Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathog Dis. 2006;3:5967. DOIPubMed
  6. Smith AM, Govender N, Keddy KH. Quinolone-resistant Salmonella Typhi in South Africa, 2003–2007. Epidemiol Infect. 2010;138:8690. DOIPubMed
  7. Hirose K, Hashimoto A, Tamura K, Kawamura Y, Ezaki T, Sagara H, DNA sequence analysis of DNA gyrase and DNA topoisomerase IV quinolone resistance–determining regions of Salmonella enterica serovar typhi and serovar paratyphi A. Antimicrob Agents Chemother. 2002;46:324952. DOIPubMed
  8. Gunell M, Webber MA, Kotilainen P, Lilly AJ, Caddick JM, Jalava J, Mechanisms of resistance in nontyphoidal Salmonella enterica strains exhibiting a nonclassical quinolone resistance phenotype. Antimicrob Agents Chemother. 2009;53:38326. DOIPubMed
  9. Pfeifer Y, Matten J, Rabsch W. Salmonella enterica serovar Typhi with CTX-M beta-lactamase, Germany. Emerg Infect Dis. 2009;15:15335. DOIPubMed
  10. Ackers ML, Puhr ND, Tauxe RV, Mintz ED. Laboratory-based surveillance of Salmonella serotype Typhi infections in the United States: antimicrobial resistance on the rise. JAMA. 2000;283:266873. DOIPubMed

Figure

Suggested citation for this article: Keddy KH, Smith AM, Sooka A, Ismail H, Oliver S. Fluoroquinolone-resistant typhoid, South Africa [letter]. Emerg Infect Dis [serial on the Internet] 2010 May [date cited]. http://wwwnc.cdc.gov/eid/article/16/5/09-1917.htm

DOI: 10.3201/eid1605.091917

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Table of Contents – Volume 16, Number 5—May 2010

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Karen H. Keddy, National Institute for Communicable Diseases, Enteric Diseases Reference Unit, Private Bag X4, Sandringham, Johannesburg 2131, South Africa





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