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Volume 16, Number 6—June 2010

Dispatch

Vaccinia Virus Infection in Monkeys, Brazilian Amazon

Jônatas S. Abrahão, André T. Silva-Fernandes, Larissa S. Lima, Rafael K. Campos, Maria I.M.C. Guedes, Marcela M.G. Cota, Felipe L. Assis, Iara A. Borges, Milton F. Souza-Júnior, Zélia I.P. Lobato, Cláudio A. Bonjardim, Paulo C.P. Ferreira, Giliane S. Trindade, and Erna G.Comments to Author 
Author affiliations: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

Main Article

Appendix Figure

Amino acid sequences of vaccinia virus (VACV) samples and comparison with homologous genes sequences from several orthopoxviruses, Brazil. A) Alignment of vaccinia growth factor gene sequences from 6 monkey serum samples showing 100% identity (horizontal box). VACV-TO_CA, sequence from Cebus apella; VACV-TO_AC, sequence from Allouata caraya; HPXV, horsepoxvirus; CPXV, cowpoxvirus; MPXV, monkeypoxvirus; VARV, variola virus; ECMV, ectromelia virus. B) Alignment of orthopoxvirus hemagglutinin gene amino acid sequences showing the deletion signature region (vertical box) in VACV-TO isolates and several VACV strains isolated during bovine vaccinia outbreaks. Arrow indicates polymorphism site in the hemagglutinin amino acid sequences between VACV-TO_CA and VACV-TO_AC. Alignments were made by using ClustalW (www.ncbi.nlm.nih.gov/pmc/articles/PMC308517) and MEGA version 3.1 software (www.megasoftware.net). HSPV, horsepoxvirus.

Appendix Figure. Amino acid sequences of vaccinia virus (VACV) samples and comparison with homologous genes sequences from several orthopoxviruses, Brazil. A) Alignment of vaccinia growth factor gene sequences from 6 monkey serum samples showing 100% identity (horizontal box). VACV-TO_CA, sequence from Cebus apella; VACV-TO_AC, sequence from Allouata caraya; HPXV, horsepoxvirus; CPXV, cowpoxvirus; MPXV, monkeypoxvirus; VARV, variola virus; ECMV, ectromelia virus. B) Alignment of orthopoxvirus hemagglutinin gene amino acid sequences showing the deletion signature region (vertical box) in VACV-TO isolates and several VACV strains isolated during bovine vaccinia outbreaks. Arrow indicates polymorphism site in the hemagglutinin amino acid sequences between VACV-TO_CA and VACV-TO_AC. Alignments were made by using ClustalW (www.ncbi.nlm.nih.gov/pmc/articles/PMC308517) and MEGA version 3.1 software (www.megasoftware.net). HSPV, horsepoxvirus.

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