Figure 2. Schematic genome organization of human astrovirus Puget Sound (HAstV-PS). Arrows represent the 3 open reading frames of the 6,584-nt single-strand, positive-sense genome. Bars above the schematic indicate the 12 contiguous fragments (contigs A–L) generated through unbiased high-throughput sequencing. PCR primers for amplification across sequence gaps were designed based on the unbiased high-throughput sequencing data, and the draft genome was resequenced by overlapping PCR products that covered the entire genome except for terminal sequences. Genomic termini were characterized with 5′ and 3′ rapid amplification of cDNA ends kits (Invitrogen, Carlsbad, CA, USA). Arrowheads indicate primer locations. PRO, protease; POL, polymerase; CA, capsid; (A)n, poly-A tail.