Volume 16, Number 6—June 2010
Research
Astrovirus Encephalitis in Boy with X-linked Agammaglobulinemia
Phenix-Lan Quan, Thor A. Wagner, Thomas Briese, Troy R. Torgerson, Mady Hornig, Alla Tashmukhamedova, Cadhla Firth, Gustavo Palacios, Ada Baisre-De-Leon, Christopher D. Paddock, Stephen K. Hutchison, Michael Egholm, Sherif R. Zaki, James E. Goldman, Hans D. Ochs, and W. Ian Lipkin
Table 6
Primers used in cloning and sequencing the human astrovirus Puget Sound genome
| Sequence (5′ → 3′) | Position |
|---|---|
| TCATGGAGCGCTCATACAAG GTGTAAGCGAAGCCAAAAGC | 38–57 815–796 |
| GATTGGGTTGAGGTTGATGC* TCCAGTGGTGGCTTGATGTA | 340–359 1797–1778 |
| GAAGTGGGATGGTGGAGTTG TCCAGAAATCCGATTCAACC | 666–685 1314–1295 |
| TGGAGCAGTTGTTGGTGAAA TTCTCAAGGTCTATCTCCCTTTGT | 1215–1234 1877–1854 |
| TACATCAAGCCACCACTGGA* TCCGCCTCTCTAAGCACTTC | 1778–1797 2159–2140 |
| TACATCAAGCCACCACTGGA TTGGATTGACTCCCTCAAGC | 1778–1797 2533–2514 |
| ATCACCACAAGAGGCGTAGG* CCAGTAACTGCTGATGGACCAACAA | 2039–2058 4649–4625 |
| CGTGATTTGCAGGAATACCA CCCATACGTGTCAGGGTTCT | 2446–2465 3343–3324 |
| TCTGGAGAGAGGCCTGATGT TCTCTCTTGACCCACATCCC | 3159–3178 3865–3846 |
| CCTCTGGGCAAATATCCACA TAGGGATATGCGGAAAACAGA | 3625–3644 4671–4651 |
| GTTTGTGGCGCTTGAAAAGT TTGCGTGGAAGAAAGTGTTG | 4583–4602 5365–5345 |
| TTGTTGGTCCATCAGCAGTTACTGG* AACTTTTGCAACCAAGCCAG | 4 625–4649 5175–5156 |
| CTGGCTTGGTTGCAAAAGTT GAGTATTGTGCCCGCAAAGT | 5156–5175 6041–6022 |
| CGGCTACACCAGCCTACATT CAGCACCATCAGACACATGG | 5833–5852 6442–6423 |
*Primer pairs designed for amplification between ultra–high-throughput sequencing contiguous fragments (Figure 2); primer pairs without asterisk were applied for resequencing of the draft genome.
