Transfer of Carbapenem-Resistant Plasmid from Klebsiella pneumoniae ST258 to Escherichia coli in Patient
Moran G. Goren, Yehuda Carmeli, Mitchell J. Schwaber, Inna Chmelnitsky, Vered Schechner, and Shiri Navon-Venezia
Author affiliations: Tel Aviv Sourasky Medical Center, Tel Aviv, Israel (M.G. Goren, Y. Carmeli, I. Chmelnitsky, V. Schechner, S. Navon-Venezia); National Center for Infection Control, Tel Aviv (Y. Carmeli, M.J. Schwaber, S. Navon-Venezia)
Figure 2. Pulsed-field gel electrophoresis demonstrating genetic relatedness of study isolates Eco2, Eco1, and Kpn1, and a representative Klebsiella pneumoniae isolate of the epidemic clone, Kpn ST258, Israel, 2008. Bacterial DNA was prepared and cleaved with 20U SpeI endonuclease (New England Biolabs, Beverly, MA, USA), followed by electrophoresis in a CHEF-DR III apparatus (Bio-Rad Laboratories, Inc., Hercules, CA, USA), as described (4). The macrorestriction patterns of the isolates were compared according to the Dice similarity index (1.5% tolerance interval) by using GelcomparII software (Applied Maths, Kortrigk, Belgium).
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