Author affiliations: National Institute of Animal Health, Tsukuba, Ibaraki, Japan (Y. Iwamaru, M. Imamura, Y. Matsuura, K. Masujin, Y. Shimizu, Y. Shu, M. Kurachi, K. Kasai, Y. Murayama, S. Mohri, H. Okada, T. Yokoyama); Hokkaido Animal Research Center, Hokkaido, Japan (S. Fukuda, S. Onoe); National Institute of Infectious Diseases, Tokyo, Japan (K. Hagiwara, Y. Yamakawa, T. Sata)
Figure 2. Bioassay using nerve tissues obtained from bovine spongiform encephalopathy JP24 prion-inoculated cattle. Inocula from selected tissues—obex (A), sciatic nerve (B), adrenal gland (C), branchial nerve plexus (D), and vagus nerve cervical part (E)—were prepared from cattle euthanized at 10 (code 8515, circle), 12 (code 498, square), and 16 (code 5566, triangle) months postinoculation were and inoculated intracerebrally into mice transgenic for bovine prion protein. Ratios above graph indicate number of prion-diseased mice/number of inoculated mice at 500 d postinoculation.
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