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Volume 16, Number 7—July 2010

Dispatch

Rickettsia felis–associated Uneruptive Fever, Senegal

Cristina Socolovschi, Oleg Mediannikov, Cheikh Sokhna, Adama Tall, Georges Diatta, Hubert Bassene, Jean-François Trape, and Didier RaoultComments to Author 
Author affiliations: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Marseille, France (C. Socolovschi, O. Mediannikov, D. Raoult); Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Dakar, Senegal (O. Mediannikov, C. Sokhna, G. Diatta, H. Bassene, J.-F. Trape); Institut Pasteur de Dakar, Dakar (A. Tall)

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Table 1

Target sequences, primers and probes used in a study of Rickettsia felis, rural Senegal, November 2008–July 2009

Quantitative real-time PCR designation and specificity Target gene Forward primer (5′ → 3′) Reverse primer (5′ → 3′) Probe
RKND03 system; Rickettsia genus-specific gltA GTG-AAT-GAA-AGA-TTA-CAC-TAT-TTA-T GTA-TCT-TAG-CAA-TCA-TTC-TAA-TAG-C 6-FAM-CTA-TTA-TGC-TTG-CGG-CTG-TCG-GTT-C-TAMRA
1029 system; Rickettsia genus-specific Hypothetical protein (RC0338 gene) GAM-AAA-TGA-ATT-ATA-TAC-GCC-GCA-AA ATT-ATT-KCC-AAA-TAT-TCG-TCC-TGT-AC 6-FAM-CTC-AAG-ATA-AGT-ATG-AGT-TAA-ATG-TAA-A-TAMRA
Rpr 331 system; specific for typhus group rickettsiae Glycosyltransferase TGC-TTC-ATG-GGC-AAT-GTC-TG TTG-AGC-ATA-AAA-CTG-CCC-TGC-T 6-FAM-CGC-TGG-ATT-ATC-AAA-AGA-ATT-AGC-ACG-TAMRA
Specific for R. felis Biotin synthase ATG-TTC-GGG-CTT-CCG-GTA-TG CCG-ATT-CAG-CAG-GTT-CTT-CAA 6-FAM- GCT-GCG-GCG-GTA-TTT-TAG-GAA-TGG-G-TAMRA
β-actin; specific for human β-actin gene Human β-actin CAT-GCC-ATC-CTG-CGT-CTG-GA CCG-TGG-CCA-TCT-CTT-GCT-CG 6-FAM-CGG-GAA-ATC-GTG-CGT-GAC-ATT-AAG-TAMRA

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