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Volume 16, Number 7—July 2010

Dispatch

Detection of Lassa Virus, Mali

David Safronetz, Job E. Lopez, Nafomon Sogoba, Sékou F. Traore’, Sandra J. Raffel, Elizabeth R. Fischer, Hideki Ebihara, Luis Branco, Robert F. Garry, Tom G. Schwan, and Heinz FeldmannComments to Author 
Author affiliations: National Institutes of Health, Hamilton, Montana, USA (D. Safronetz, J.E. Lopez, S.J. Raffel, E.R. Fischer, H. Ebihara, T.G. Schwan, H. Feldmann); University of Bamako, Bamako, Mali (N. Sogoba, S.F. Traore’); Tulane School of Medicine, New Orleans, Louisiana, USA (L. Branco, R.F. Garry); Autoimmune Technologies LLC, New Orleans (L. Branco)

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Figure 2

Phylogenetic analysis of Lassa virus conducted on A) a 754-bp fragment of the polymerase gene (large genomic segment nucleotide positions 3427–4180) and B) a 771-bp fragment of the glycoprotein precursor (small genomic segment nucleotide positions 2526–3296). The fragments were amplified from infected rodent tissues with sequence analysis accomplished with PHYLIP version 3.69 software (http://evolution.genetics.washington.edu/phylip.html) by using the neighbor-joining method with 1,000 replicate

Figure 2. Phylogenetic analysis of Lassa virus conducted on A) a 754-bp fragment of the polymerase gene (large genomic segment nucleotide positions 3427–4180) and B) a 771-bp fragment of the glycoprotein precursor (small genomic segment nucleotide positions 2526–3296). The fragments were amplified from infected rodent tissues with sequence analysis accomplished with PHYLIP version 3.69 software (http://evolution.genetics.washington.edu/phylip.html) by using the neighbor-joining method with 1,000 replicates of bootstrap. Sequences were compared with the following arenavirus sequences (small and large segment GenBank accession nos.): Tacaribe (NC_004293, NC_004292), Lujo (NC_012776, NC_012777), Lymphocytic choriomeningitis virus (LCMV, strain Armstrong, AY847350,J04331), Ippy (NC_007905, NC_007906), Mobala (NC_007903, NC_007904), Morogoro (NC_013057, NC_013058), Mopeia (NC_006575, NC_006574), and Lassa virus strains 803213 (AF803213, AY693640), CSF (AF333969, AY179174), NL (AY179173, AY179172), Josiah (AY628203, U63094), Z148 (AY628205, AY628204), Macenta (AY628201, AY628200), and AV (AF246121, AY179171). Small segment analysis also included Lassa virus strains LP (AF18185), Weller (AY628206), Nigeria (M36544), the sequence generated from the imported case of Lassa fever from Mali (FJ824031), and Junin virus (NC_005081). Bootstrap values >800 are shown. *Whether the AV strain of Lassa virus originated from Côte d’Ivoire, Burkina Faso, or Ghana is not clear. Italicized names represent sequences generated as part of these studies. Scale bars indicate 5% (A) or 20% (B) nucleotide divergence.

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