Author affiliations: CIBIO-UP (Centro de Investigação em Biodiversidade e Recursos Genéticos, Vairão–Universidade do Porto, Porto, Portugal (J. Abrantes, P.J. Esteves); Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 892, Université de Nantes, Nantes, France (J. Abrantes); CESPU (Cooperativa de Ensino Superior Politécnico e Universitário), Gandra, Portugal (P.J. Esteves).
Figure. Evolutionary relationships of Lagovirus strains. The evolutionary history was inferred by using the neighbor-joining method (3) with the pairwise deletion option. The tree is drawn to scale. There were a total of 563 positions (97% of the capsid viral protein [60 aa sequence]). Phylogenetic analyses were conducted in MEGA 4 (4). Reliability of the tree was assessed by bootstrap with 1,000 replicates and is indicated in the nodes (only relevant values are shown). Several genetic distance methods were used, and similar results were obtained, but only p-distance is shown. GenBank accession numbers of the sequences used are indicated. Scale bar indicates nucleotide substitutions per site. RDHV, rabbit hemorrhagic disease virus; RCV, rabbit calicivirus; EBHSV, European brown hare syndrome virus.
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