Volume 16, Number 8—August 2010
Research
Bat Coronaviruses and Experimental Infection of Bats, the Philippines
Shumpei Watanabe, Joseph S. Masangkay, Noriyo Nagata, Shigeru Morikawa, Tetsuya Mizutani, Shuetsu Fukushi, Phillip Alviola, Tsutomu Omatsu, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Hikaru Fujii, Shumpei Tsuda, Maiko Endoh, Kentaro Kato, Yukinobu Tohya, Shigeru Kyuwa, Yasuhiro Yoshikawa, and Hiroomi Akashi
Table 2
PCR results for bat coronavirus in fruit bats infected by using bat intestinal samples, the Philippines*
| Bat | Assay | Liver | Kidney | Lung | Spleen | Brain | Small intestine | Large intestine | Serum |
|---|---|---|---|---|---|---|---|---|---|
| A | RT-PCR | – | – | – | – | – | – | + | – |
| qRT-PCR |
ND |
ND |
ND |
ND |
ND |
1.25 × 106 |
3.53 × 106 |
ND |
|
| B | RT-PCR | – | – | – | – | – | + | + | – |
| qRT-PCR | ND | ND | ND | ND | ND | 1.47 × 106 | 1.50 ×106 | – |
*Bat intestinal samples containing 7.8 × 106 copies of coronavirus genome. Values are copies per milligram. RT-PCR, reverse transcription–PCR; –, virus RNA not detected; +, virus RNA detected; qRT-PCR, quantitative RT-PCR; ND, not done.
