Volume 16, Number 8—August 2010
Research
Bat Coronaviruses and Experimental Infection of Bats, the Philippines
Shumpei Watanabe, Joseph S. Masangkay, Noriyo Nagata, Shigeru Morikawa, Tetsuya Mizutani, Shuetsu Fukushi, Phillip Alviola, Tsutomu Omatsu, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Hikaru Fujii, Shumpei Tsuda, Maiko Endoh, Kentaro Kato, Yukinobu Tohya, Shigeru Kyuwa, Yasuhiro Yoshikawa, and Hiroomi Akashi
Table 4
Results of nested and quantitative RT-PCRs of cDNA from bat samples, the Philippines*
| Bat | Liver | Kidney | Lung | Spleen | Brain | Small intestine | Large intestine | Serum | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| C | – | – | – | – | – | + (ND) | – | – | ||||
| D | – | + (ND) | + (ND) | + (ND) | – | + (6.57 × 104)† | – | + (ND) | ||||
| E | – | – | – | – | – | – | – | – | ||||
| Intestine section |
||||||||||||
| 1 |
2 |
3 |
4 |
5 |
6 |
|||||||
| F | – | – | – | – | – | – | – | + (ND) | + (ND) | – | – | – |
| G | – | – | – | – | – | – | – | + (ND) | + (ND) | + (ND) | + (5.91 × 104) | – |
*cDNA was synthesized from bat samples obtained after experimental infection (second passage of the group 2d coronavirus in Leschenault rousette bats). Values are copies per milligram. Virus load was quantified by real-time PCR. RT-PCR,reverse transcription–PCR; –, virus RNA not detected; +, virus RNA detected; ND, not detected by real-time PCR in RT-PCR–positive samples.
†Result of nested PCR.
