Volume 16, Number 9—September 2010
Analysis of Avian Hepatitis E Virus from Chickens, China
|Primer*||Sequence, 5′ → 3′†||Position, nt‡|
*Primers F1-1 to R1-2, F2-1 to R2-2, F3-1 to R3-2, and F4-1 to R4-2 were used to amplify the first, second, third, and fourth fragment of the near-complete avian hepatitis E virus (HEV) genome. Primers F5, amplification primer (AUAP), and adapter primer (AP) were used to amplify the extreme 3′ genomic sequence. Primers R1-1, R2-1, R3-1, R4-1, and AP are also reverse transcription primers.
†Sequences of primers were designed according to the sequences of 4 other known avian HEV strains.
‡Positions of primers located in the complete genome are shown according to the Europe avian HEV isolate.
§Commercial primer (Invitrogen, Carlsbad, CA, USA) of nonviral origin.
1Current affiliation: Northwest A&F University, Yangling, People’s Republic of China.