Volume 17, Number 1—January 2011
Dispatch
New Delhi Metallo-β-Lactamase in Klebsiella pneumoniae and Escherichia coli, Canada
Michael R. Mulvey
, Jennifer M. Grant, Katherine Plewes, Diane Roscoe, and David A. Boyd
, Jennifer M. Grant, Katherine Plewes, Diane Roscoe, and David A. BoydTable 1
Primers used in study of New Delhi metallo-β-lactamase in Klebsiella pneumoniae and Escherichia coli, Canada, 2010
| Name | Sequence, 5′ → 3′* | Product size, bp |
|---|---|---|
| SHV-UP | CGCCGGGTTATTCTTATTTGTCGC | 1,016 |
| SHV-LO | TCTTTCCGATGCCGCCGCCAGTCA | |
| TEM-G | TTGCTCACCCAGAAACGCTGGTG | 708 |
| TEM-H | TACGATACGGGAGGGCTTACC | |
| CTX-U1 | ATGTGCAGYACCAGTAARGTKATGGC | 593 |
| CTX-U2 | TGGGTRAARTARGTSACCAGAAYCAGCGG | |
| OXA1-F | CGCAAATGGCACCAGATTCAAC | 464 |
| OXA1-R | TCCTGCACCAGTTTTCCCATACAG | |
| CMY2-A | TGATGCAGGAGCAGGCTATTCC | 323 |
| CMY2-B | CTAACGTCATCGGGGATCTGC | |
| KPC1 | ATGTCACTGTATCGCCGTC | 863 |
| KPC2 | AATCCCTCGAGCGCGAGT | |
| VIM1 | GTTTGGTCGCATATCGCAAC | 382 |
| VIM2 | AATGCGCAGCACCAGGATAGAA | |
| IMP1 | CCWGATTTAAAAATYGARAAGCTTG | 522 |
| IMP2 | TGGCCAHGCTTCWAHATTTGCRTC | |
| NDM-F | GGTGCATGCCCGGTGAAATC | 660 |
| NDM-R | ATGCTGGCCTTGGGGAACG | |
| NDM-A | CACCTCATGTTTGAATTCGCC | 984 |
| NDM-B | CTCTGTCACATCGAAATCGC |
*H is A, C, or T; K is G or T; R is A or G; S is G or C; and W is A or T.
