Volume 17, Number 10—October 2011
Perspective
Global Spread of Carbapenemase-producing Enterobacteriaceae
, Thierry Naas, and Laurent PoirelTable 3
Oligonucleotides used for screening of main carbapenemase genes in Enterobacteriaceae*
| Primer | Sequence, 5′ → 3′ | Gene | Product size, bp |
|---|---|---|---|
| IMP-F | GGAATAGAGTGGCTTAAYTC | blaIMP | 232 |
| IMP-R | TCGGTTTAAYAAAACAACCACC | ||
| VIM-F | GATGGTGTTTGGTCGCATA | blaVIM | 390 |
| VIM-R | CGAATGCGCAGCACCAG | ||
| OXA-48-F | GCGTGGTTAAGGATGAACAC | blaOXA-48 | 438 |
| OXA-48-R | CATCAAGTTCAACCCAACCG | ||
| NDM-F | GGTTTGGCGATCTGGTTTTC | blaNDM | 621 |
| NDM-R | CGGAATGGCTCATCACGATC | ||
| KPC-Fm | CGTCTAGTTCTGCTGTCTTG | blaKPC | 798 |
| KPC-Rm | CTTGTCATCCTTGTTAGGCG |
*A detailed technique for PCR amplification has been reported by Poirel et al. (34). VIM, Verona integron–encoded metallo-β-lactamase; OXA, oxacillinase; NDM, New Delhi metallo-β-lactamase-1; KPC, Klebsiella pneumoniae carbapenemase. *A detailed technique for PCR amplification has been reported by Poirel et al. (34). VIM, Verona integron–encoded metallo-β-lactamase; OXA, oxacillinase; NDM, New Delhi metallo-β-lactamase-1; KPC, Klebsiella pneumoniae carbapenemase. |
*A detailed technique for PCR amplification has been reported by Poirel et al. (34). VIM, Verona integron–encoded metallo-β-lactamase; OXA, oxacillinase; NDM, New Delhi metallo-β-lactamase-1; KPC, Klebsiella pneumoniae carbapenemase.
